(Z)-cyclooctene

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non guideline, no GLP

Data source

Reference

Reference Type:

publication

Title:

The Metabolism of Cyclo-octene, 1, 3-cyclo-octadiene and 1, 5-cyclo-octadiene in the Rat and Rabbit, and the Reaction of these Compounds with Glutathione in vivo in the Rat

Author:

Waring, R.H.

Year:

1971

Bibliographic source:

Xenobiotica 1, 303-308

Materials and methods

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cyclooctene

EC Number:

213-245-5

EC Name:

Cyclooctene

Cas Number:

931-88-4

IUPAC Name:

cyclooctene

Details on test material:

- Name of test material (as cited in study report): cyclooctene

Test animals

Species:

other: rats and rabbits

Strain:

not specified

Sex:

female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

not specified

Duration and frequency of treatment / exposure:

single dose

Doses / concentrations

Remarks:

Doses / Concentrations:
2.0 mmol/kg bw

No. of animals per sex per dose / concentration:

6

Control animals:

no

Results and discussion

Metabolite characterisation studies

Metabolites identified:

yes

Details on metabolites:

Two sulfur containing metabolites were detected in urine of both female rats and rabbits following a single dose of cyclooctene.
These were identified as 2-hydroxycyclo-octyl mercapturic acid and 3-hydoxycyclo-octyl mercapturic acid by co-chromatography with synthetic standards and mass spectrometry.
Cis/trans isomers were present in 1:1 ratio.

Any other information on results incl. tables

Sulfur containing metabolites in urine (24h)

	Dose (mmol/kg bw)	Female Rabbit (n=6)				Female Rat (n=6)
		Glucuronide (% of dose)	Ethereal sulphate (% of dose)	Mercapturic acids (% of dose)		Mercapturic acids (% of dose)
				2-Hydroxy-	3-Hydroxy-	2-Hydroxy-	3-Hydroxy-
Cyclooctene	2.0	51.2	20.3	2.3	8.3	4.9	7.2

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): no bioaccumulation potential based on study results
More than 80% of an oral dose of 2 mmol cyclooctene (220mg)/kg bw was excreted within 24h as sulfur containing metabolites indicating extensive absorption and extensive metabolism including hydroxylation as well as rapid excretion.

Executive summary:

In a published study the formation of sulfur containing metabolites in female rabbits and rats was investigated.

Animals received a single dose of 2 mmol (220mg) per kg bw, 24h urine was collected and analysed. More than 80% of the dose was recovered as sulfur containing metabolites of which 2-hydroxy and 3-hydroxy-mercapturic acids were identified.