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EC number: 213-243-4 | CAS number: 931-87-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-08-30 to 1990-09-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions: TA 102 or E.coli WP2 were not tested (not required by applied version of guideline).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Version / remarks:
- Cited as Directive 84/449/EEC, B.14
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Cyclooctene
- EC Number:
- 213-245-5
- EC Name:
- Cyclooctene
- Cas Number:
- 931-88-4
- IUPAC Name:
- cyclooctene
- Details on test material:
- Cyclooctene-cis of Hüls AG, purity 96.81 %, produced 01 Mar 1990, ID 3641/81173
Constituent 1
Method
- Target gene:
- mutated gene loci responsible for histidine auxotrophy
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA 1535, TA 1537, TA 1538, TA 98, TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat S9 liver
- Test concentrations with justification for top dose:
- 8; 40; 200; 1000; 5000 µg/plate
- Vehicle / solvent:
- acetone
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- sodium azide for TA 100 and 1535, aminoacridine for TA 1537
Migrated to IUCLID6: TA 98 and 1538
- Details on test system and experimental conditions:
- Ames test
SYSTEM OF TESTING
- Metabolic activation system: Aroclor 1254 induced rat S9 liver, male Bor: WISW (SPF/Cpb) rats
ADMINISTRATION:
- Dosing:
main test: 8/40/200/1000/5000 µg/plate (+/- metabolic activation)
pre-incubation test: 8/40/200/1000/5000 µg/plate (+/- metabolic activation)
- Number of replicates: 3
- Application: solvent acetone (CAS No. 67-64-1) main test 100 g/l, pre-incubation test 200 g/l
- Positive and negative control groups and treatment:
positive, TA 98 and TA 1538: nitrofluorene
positive, TA 100 and TA 1535: sodium azide
positive, TA 1537: aminoacridine
negative: solvent + untreated controls activity of metabolic system: aminoanthracene / TA 100
- Pre-incubation: 30 minutes at 30 °C incubation 96 hours at 37 °C (approximately) - Evaluation criteria:
- CRITERIA FOR EVALUATING RESULTS: mutagenic effects (i.e ratio of revertant rates treated/control >= 2) at <= 5000 µg/plate with generally
positive dose-response relationship in any strain - Statistics:
- usual statistical methods (standard deviation; means; factors) were calculated through a computer program by Messrs BIOSYS.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: Salmonella typhimurium TA 1535, TA 1537, TA 1538, TA 98, TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: 5000 µg/plate in TA 1535 and TA 1537, only without S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- GENOTOXIC EFFECTS:
- With metabolic activation: None
- Without metabolic activation: None
The positive controls were functional.
PRECIPITATION CONCENTRATION:
5000 µg/plate in pre-incubation test
CYTOTOXIC CONCENTRATION (including effects on background lawn):
TA 98: none (+/- S9)
TA 100: none (+/- S9)
TA 1535: 5000 µg/plate (- S9)
TA 1537: 5000 µg/plate (- S9)
TA 1538: none (+/- S9) - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Cis-cyclooctene was not mutagenic when tested on S. typhimurium strains TA98, TA100, TA1535, TA1537 and TA1538, with or without S9-mix activation. - Executive summary:
In a reverse gene mutation assay in bacteria, strains TA98, TA100, TA1535, TA1537 and TA1538 of Salmonella typhimurium were exposed to Cis-cyclooctene using acetone as a vehicle at concentrations of 8, 40, 200, 100 and 5000 µg/plate, with and without S-9 activation in a preincubation assay according to EEC guidance Dir. 84/449/EEC, B.14.
The test article was tested at six dose levels along with appropriate vehicle and positive controls on the tester strains mentioned above in the presence and absence of S9-mix. All dose levels, vehicle and positive controls were plated in triplicate.
Cytotoxicity was noted in the absence of S9 mix at the highest concentration for TA 1535 and TA1537.
No positive mutagenic responses were observed with any of the strains used, in the presence as well as in the absence of microsomal enzymes. These results were confirmed in an independent assay.
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