Ethyl diphenylphosphinite

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 "Testverfahren mit Wasserorganismen (Gruppe L)."

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl diphenylphosphinite

EC Number:

211-951-8

EC Name:

Ethyl diphenylphosphinite

Cas Number:

719-80-2

Molecular formula:

C14H15OP

IUPAC Name:

ethyl diphenylphosphinite

Details on test material:

- Name of test substance (as cited in study report): Ethosydiphenylphosphin
- Batch No.: 890011
- Purity: 94.2 %
- Homogeneity: ensured since the test substance is a genuine liquid
- Physical state: liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

- Common name: Golden orfe
- Supplier: Fischzucht Paul Eggers, Hohenwestedt, Germany
- Body length: 8.4 cm (7.5 - 9.3 cm)
- Body weight: 6.0 g (4.4 - 7.6 g)
- Housing: Fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air
Photoperiod: 16 h light : 8 h dark
Water temperature: 18 - 22°C
- Water parameters of housing water: - total hardness: about 2.5 mmol/L
- acid capacity: about 5.5 mmol/L
- oxygen content: > 60% of max. saturation
- pH: about 8.0
- Duration of housing and adaptation: 4 months
- Mortality during last 2 weeks of housing: about 0.1%
- Mortality during adaptation period: 0%
- Medical treatment: twice with 0.05 mg/L malachite green chloride
once with 10 mg/L tetracycline hydrochloride
- Diet: growing feed F/B 50, SSNIFF Spezialdiaeten GmbH, Soest, Germany
frequency: ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

22 +/- 1°C

pH:

7.0 - 7.5

Dissolved oxygen:

6.9 - 8.1 mg/L

Nominal and measured concentrations:

- Test concentrations (nominal): 4.64, 10, 21.5, 46.4 and 100 mg/L

Details on test conditions:

TEST WATER
- Reconstituted freshwater according to DIN 38412, part 11
- Preparation from fully demineralized tap water
- Conductivity: max. 10 micro mho
- Resalting: - 294.0 mg/L CaCl2 * 2 H2O
- 123.3 mg/L MgSO4 * 7 H2O
- 63.0 mg/L NaHCO3
- 5.5 mg/L KCl
- Aeration: continuous with oil-free air
- Test water ready for use:
- Acid capacity: 0.8 mmol/L
- Ratio Ca/Mg-Ions: 4:1
- Ratio Na/K-Ions: 10:1
- pH: about 8.0

TEST SYSTEM
- Test vessel: all-glass aquarium (30 x 22 x 24)
- Volume of water: 10 L
- No. of animals per test concentration: 10
- Loading (g fish / L test water): 6.0

FURTHER CONDITIONS
- Adaptation to test water and test temperature: 3 days
- Withdrawl of food: 1 day before and during exposure

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 21.5 - < 46.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Visual observations: the aquarium contents were increasingly cloudy with increasing concentration