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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1982
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Version / remarks:
adopted May 12th, 1981
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Diphenylethoxyphosphin
IUPAC Name:
Diphenylethoxyphosphin
Constituent 2
Chemical structure
Reference substance name:
Ethyl diphenylphosphinite
EC Number:
211-951-8
EC Name:
Ethyl diphenylphosphinite
Cas Number:
719-80-2
Molecular formula:
C14H15OP
IUPAC Name:
ethyl diphenylphosphinite
Details on test material:
- Name of test material (as cited in study report): Diphenylethoxyphospin
- physical state: liquid
- Analytical purity: no data
- Expiration date of the lot/batch: October 1982

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: Gaukler, Offenbach/Main
- Age at study initiation: no data
- Weight at study initiation: male: 3.13kg, females 3.3kg, 3.27kg
- Housing: single in steel cages (40cm x 51cm)
- Diet (e.g. ad libitum): Ovator Solikanin 4mm, app. 130g per animal and day
- Water (e.g. ad libitum): app. 250ml per animal and day (VE water on weekdays, tap water on weekends)
- Acclimation period: app. 8 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-26°C
- Humidity (%):app. 30-70%
- Photoperiod (hrs dark / hrs light): 12h/12h

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eye of the same animal
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1ml
Observation period (in vivo):
72h (1h, 24h, 48h, 72h after application)
Number of animals or in vitro replicates:
1 male, 2 females
Details on study design:
REMOVAL OF TEST SUBSTANCE
- substance not washed out

SCORING SYSTEM: according to Draize

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24h-72h
Score:
0
Max. score:
4
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24h-72h
Score:
0
Max. score:
2
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24h-72h
Score:
0
Max. score:
4
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24h
Score:
1
Max. score:
3
Reversibility:
fully reversible within: 72h
Remarks on result:
other: slight discharge in animal no.1
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 48h
Score:
1.3
Max. score:
3
Reversibility:
fully reversible within: 72h
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 72h
Score:
0
Max. score:
3

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information