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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
biochemical or cellular interactions
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Effects of cyclic 12-, 8- and 6-carbon compounds on glutathione S-transferase activity.
Author:
Sparnins VL, Lam LKT and Wattenberg LW
Year:
1984
Bibliographic source:
Biochem. Biophys. Res. Commun. 120, 637-640.

Materials and methods

Principles of method if other than guideline:
see reference
Type of method:
in vivo
Endpoint addressed:
basic toxicokinetics

Test material

Constituent 1
Reference substance name:
(1E,5E,9Z)-cyclododeca-1,5,9-triene
EC Number:
211-893-3
EC Name:
(1E,5E,9Z)-cyclododeca-1,5,9-triene
Cas Number:
706-31-0
IUPAC Name:
cyclododeca-1,5,9-triene
Constituent 2
Reference substance name:
trans-trans-cis-1,5,9-cyclododecatriene
IUPAC Name:
trans-trans-cis-1,5,9-cyclododecatriene
Details on test material:
trans-trans-cis-1,5,9-cyclododecatriene (CAS RN 706-31-0) from Aldrich Chemical Company, purity not reported

Test animals

Species:
mouse
Strain:
other: ICR/Ha mice
Sex:
female
Details on test animals or test system and environmental conditions:
Age: 7 weeks
Number: 5-10 animals per group

Administration / exposure

Route of administration:
oral: feed
Details on exposure:
Control: diet
TREATMENT
- 30 or 50 µmol/g in diet for 2 weeks
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
2 weeks
Frequency of treatment:
test item in feed
Doses / concentrations
Remarks:
Doses / Concentrations:
30 or 50 µmol/g in diet
Basis:
nominal in diet
No. of animals per sex per dose:
Number: 5-10 animals per group
Control animals:
yes

Examinations

Examinations:
EXAMINATIONS
- Removal and homogenization of liver, forestomach, and mucosa from small  bowel
- GST activity determination using 1-chloro-2,4-dinitrobenzene as  substrate

Results and discussion

Details on results:
None of the compounds elicited increased GST activity in the forestomach.  C6 ring compounds showed no significant effect. C12 ring compounds were  more effective than C8 ring compounds with a decrease in activity in the  order: unsaturated > epoxide > alcohol > saturated. With trans-trans-cis-1,5,9-cyclododecatriene as test substance, the GST  activity in the livers was increased 3.79-, 3.78- and 3.82-fold,  respectively, in three experiments. The increases in the intetinal GST  activity were of similar magnitude.

Any other information on results incl. tables


-----------------------------------------------------------
Test compound          Liver              Small Bowel Mucosa
                    Specific Activity      Specific Activity
------------------------------------------------------------
None                1.96 +- 0.16           0.61 +- 0.04
Cyclododecatriene   7.42 +- 0.53*          2.77 +- 0.29*

None                2.40 +- 0.10           0.65 +- 0.01
Cyclododecatriene   9.06 +- 0.04*          1.74 +- 0.15*

None                1.39 +- 0.05           0.27 +- 0.01
Cyclododecatriene   5.31 +- 0.23*          1.10 +- 0.09*
     * = p <0.005

Applicant's summary and conclusion

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