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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Specific investigations: other studies

Currently viewing:

Administrative data

Endpoint:
biochemical or cellular interactions
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Effects of cyclic 12-, 8- and 6-carbon compounds on glutathione S-transferase activity.
Author:
Sparnins VL, Lam LKT and Wattenberg LW
Year:
1984
Bibliographic source:
Biochem. Biophys. Res. Commun. 120, 637-640.

Materials and methods

Principles of method if other than guideline:
see reference
Type of method:
in vivo
Endpoint addressed:
basic toxicokinetics

Test material

Constituent 1
Reference substance name:
(1E,5E,9Z)-cyclododeca-1,5,9-triene
EC Number:
211-893-3
EC Name:
(1E,5E,9Z)-cyclododeca-1,5,9-triene
Cas Number:
706-31-0
IUPAC Name:
cyclododeca-1,5,9-triene
Constituent 2
Reference substance name:
trans-trans-cis-1,5,9-cyclododecatriene
IUPAC Name:
trans-trans-cis-1,5,9-cyclododecatriene
Details on test material:
trans-trans-cis-1,5,9-cyclododecatriene (CAS RN 706-31-0) from Aldrich Chemical Company, purity not reported

Test animals

Species:
mouse
Strain:
other: ICR/Ha mice
Sex:
female
Details on test animals or test system and environmental conditions:
Age: 7 weeks
Number: 5-10 animals per group

Administration / exposure

Route of administration:
oral: feed
Details on exposure:
Control: diet
TREATMENT
- 30 or 50 µmol/g in diet for 2 weeks
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
2 weeks
Frequency of treatment:
test item in feed
Doses / concentrations
Remarks:
Doses / Concentrations:
30 or 50 µmol/g in diet
Basis:
nominal in diet
No. of animals per sex per dose:
Number: 5-10 animals per group
Control animals:
yes

Examinations

Examinations:
EXAMINATIONS
- Removal and homogenization of liver, forestomach, and mucosa from small  bowel
- GST activity determination using 1-chloro-2,4-dinitrobenzene as  substrate

Results and discussion

Details on results:
None of the compounds elicited increased GST activity in the forestomach.  C6 ring compounds showed no significant effect. C12 ring compounds were  more effective than C8 ring compounds with a decrease in activity in the  order: unsaturated > epoxide > alcohol > saturated. With trans-trans-cis-1,5,9-cyclododecatriene as test substance, the GST  activity in the livers was increased 3.79-, 3.78- and 3.82-fold,  respectively, in three experiments. The increases in the intetinal GST  activity were of similar magnitude.

Any other information on results incl. tables


-----------------------------------------------------------
Test compound          Liver              Small Bowel Mucosa
                    Specific Activity      Specific Activity
------------------------------------------------------------
None                1.96 +- 0.16           0.61 +- 0.04
Cyclododecatriene   7.42 +- 0.53*          2.77 +- 0.29*

None                2.40 +- 0.10           0.65 +- 0.01
Cyclododecatriene   9.06 +- 0.04*          1.74 +- 0.15*

None                1.39 +- 0.05           0.27 +- 0.01
Cyclododecatriene   5.31 +- 0.23*          1.10 +- 0.09*
     * = p <0.005

Applicant's summary and conclusion