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Description of key information

Acute oral toxicity: A reliable study (OECD 401) is available. Oral LD50 is equal to 4400 mg/kg bw in rats.
Acute inhalation toxicity : Two reliable study are available, test item was tested as aerosol in rats. LC50 (4) > 8.1 mg/L.
Acute dermal toxicity: A data is available but this study is not assignable because data is unpublished. This study showed a dermal LD50 > 3520 mg/kg in rabbits.

Key value for chemical safety assessment

Acute toxicity: via oral route

Endpoint conclusion
Dose descriptor:
LD50
Value:
4 400 mg/kg bw

Acute toxicity: via inhalation route

Endpoint conclusion
Dose descriptor:
LC50
Value:
8.1 mg/m³

Acute toxicity: via dermal route

Endpoint conclusion
Dose descriptor:
LD50
Value:
3 520 mg/kg bw

Additional information

Acute toxicity : oral

In a determination of the acute oral toxicity on male and female rats it was found that the LD50 of the test item 1,5,9-cyclododecatriene is 4400 mg/kg bw. 30 minutes after application animals showed restlessness, rough skin, decrease of responsiveness, hunched   posture, diarrhoea, moderate to severe tremors, convulsions, sedation, prone position. Those animals surviving the test appeared normal after 5 days at the latest. There was practically no influence on the increase in body weight. Dissection at the end of the experiment revealed redness of the mucosa of the gut and stomach and an accumulation of liquor. Animals which survived the study showed no pathological changes.Under the conditions of this study the acute toxicity of 1,5,9-cyclododecatriene after oral application in rats is very low.

Acute toxicity : inhalation

In the first reliable study, two groups of 6 male rats each were exposed nose-only for a single, 4-hour periode to vapors of the test item 1,5,9 -Cyclododecatriene in air at chamber vapor concentrations of 6.1 or 8.1 mg/l. Mortality was 1/6 in the high dose group. Clinical signs noted in rats from both exposures included included ocular and nasal discharges, irregular respiration, abnormal gait or mobility, and tremors. Additionally, the rats exposed to  8.1 mg/l exhibited aggressive behavior and vocalization. Tremors were observed only on test day 1 in both groups. Abnormal gait or mobility was observed only on test day 1 in rats exposed to 6.1 mg/l and up to 3 days following exposure in rats exposed to  8.1 mg/l. No clinical signs of toxicity were observed after day 4 following exposure.

Therefore it is concluded that the LC50 for male rats is greater than 8.1 mg/l air for the test item 1,5,9-cyclododecatriene under the conditions of the study.

In the second study, male rats were exposed whole body for a single, 6 hour periode to vapors of the test item 1,5,9-cyclododecatriene in air at chamber vapor concentrations of 5.0, 6.0, 7.0, 8.0, 8.5, 9.0 and10.0 mg/l. Mortality was 0/10; 1/10; 5/10; 5/20; 4/10; 3/10; 18/20 within the exposure groups (5.0, 6.0, 7.0, 8.0, 8.5, 9.0 and 10.0 mg/l) and 92 % died during exposure. Clinical signs noted in rats from all exposures included gasping, twiching, and severe muscle spasms.

In general, recovery of the animals was complete by the third day following exposure. Under the conditions of the study it is concluded that the LC50 for male rats is 8.2 mg/l (95% confidence interval = 7.5 - 8.9) for the test item 1,5,9-cyclododecatriene.

Acute toxicity : dermal

Two rats of each sex (aged 12 -13 weeks) were used at each dose level (2, 3 or 4 mL/kg). The test substance was placed onto the shorn dorso-lumbar skin, and bandaged to contact the skin using an impermeable dressing of aluminium foil and waterproof plaster. The rats were housed individually over the 24 -hour exposure period, during which time they were deprived of food, but allowed water ad libitum. After 24 hours, the dressings were removed and the exposed area was washed with a tepid dilute detergent solution. The rats were then housed 3/cage, egnders separate, and observed for signs of intoxiciation during the following 9 days. None of the rabbits died during the test. All the rats had eschar on their backs when the occlusive dressing had been removed.

Under the conditions of this study the acute toxicity of 1,5,9-cyclododecatriene after dermal application in rabbits is very low with a LD50 higher than 3520 mg/kg (4 mL/kg).

Justification for classification or non-classification

Proposed self-classification

- Regulation (EC) No 1272/2008

Not classified

- Directive 67/548/EEC

Not classified

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