Coconut oil, reaction products with polyethylene glycol and trimethylolpropane

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11/1996 - 02/1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Principles of method if other than guideline:

according to EG 92/69 C1 and OECD guideline 203

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Zierkarpfen
- Strain: Cyprinus carpio
- Source: Charles River, Someren, NL
- Age at study initiation (mean and range, SD): not mentioned
- Length at study initiation (length definition, mean, range and SD): 2-3 cm, no further details mentioned
- Weight at study initiation (mean and range, SD):
- Method of breeding: not mentioned
- Feeding during test: none


ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): The fish were kept in 200 L earthenware basins, or in 300 L glass aquariums in flow rate (20 +- 2°C).
- Type and amount of food: Trouvit, administration in an amount of approx. 1 % of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): The animals showed a normal behaviour pattern at the beginning of the test and were free from visible diseases. The mortality rate during the holding phase was < 5% seven days before the beginning of the test.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

none

Hardness:

11 °dH

Test temperature:

19.6 - 20.1 °C

pH:

7.4 - 8.4

Dissolved oxygen:

90 - 99 % - saturation

Nominal and measured concentrations:

Nominal: 2; 9.9; 98.6; 986 mg/L
measured: 1. meaurement: 1.2; 9.9; 70; 738 mg/L; 2. measurement: 6.3; 12.5; 88; 951 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): not mentioned
- Material, size, headspace, fill volume: 20 L, 10 L fill volume, no further details mentioned
- Aeration: continously, no further details mentioned
- Renewal rate of test solution (frequency/flow rate): daily renewal
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.6 g/fish


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water, Gelsenwasser AG, Germany
no further details mentioned
- Intervals of water quality measurement: not mentioned

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity: not mentioned


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality after 24, 48, 72 and 96 hours; pH, temperature, O2 saturation at the end of the test


TEST CONCENTRATIONS
- Spacing factor for test concentrations: not mentioned
- Range finding study: not mentioned

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

123 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

50 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

202 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The testsubstance ist due to its properties insoluble in water. The concentrations employed were in all cases emulsions. All concentrations are based on the product wihout water.

Results with reference substance (positive control):

no reference substance tested

Reported statistics and error estimates:

no statistics performed, the LC50 values were calculated graphically (dose-response relationship).

Sublethal observations / clinical signs:

concentration[mg/L]	No. of fish at start of study	observation period
		24 h		48 h		72 h		96 h
		No. dead	mortality [%]	No. dead	mortality[%]	No. dead	mortality[%]	No. dead	mortality[%]
control	10	0	0	0	0	0	0	0	0
50	10	0	0	0	0	0	0	0	0
101	10	0	0	0	0	1	10	3	30
202	10	0	0	10	100	10	100	10	100
403	10	10	100	10	100	10	100	10	100
806	10	10	100	10	100	10	100	10	100

Validity criteria fulfilled:

yes

Executive summary:

The substance Coconut oil, reaction products with polyethylene glycol and trimethylolpropane was investigated in a semi-static test according to 92/69/EEC; part C 1.

After exposure of Cyprinus carpio for 96 h, the highest concentration at which no mortality (<=10 %) occurred was LC0 = 50 mg/L, the LC100 = 202 mg/L. The LC50 after 96 h was 123 mg/L.

Description of key information

The substance Coconut oil, reaction products with polyethylene glycol and trimethylolpropane was investigated in a semi-static test according to 92/69/EEC; part C 1.

After exposure of Cyprinus carpio for 96 h, the highest concentration at which no mortality (<=10 %) occurred was LC0 = 50 mg/L, the LC100 = 202 mg/L. The LC50 after 96 h was 123 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

123 mg/L