Coconut oil, reaction products with polyethylene glycol and trimethylolpropane

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22.10.1996 - 24.10.1996

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: 92/69/EWG

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

stock solution 1 g/L in VE-water

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna Straus, clone 5
- Source: laboratory breed
- Age at study initiation: not older than 24 hours
- Method of breeding: The females were maintained in M4 medium in 1 L beakers. A reddish dark-brown coloration of the animals indicated that they were healthy. At two to three day intervals, the exuviae were siphoned off and the water was changed. In the course of this, the offspring was removed from the breeding vessels. At regular intervals of approx. 4 weeks, offspring was isolated from the vessels. They served as a basis for further breeding.

ACCLIMATION
- Acclimation period: Females were seperated from the offspring 24 hours before the start of the test. The offspring produced overnight were transferred into test water and were used for the test.
- Type and amount of food: Scenedesmus subspicatus, the amount of food added is such that it is consumed by the animals
- Feeding frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Hardness:

not mentioned

Test temperature:

20 °C ± 1 °C

pH:

not mentioned

Dissolved oxygen:

not mentioned

Nominal and measured concentrations:

Nominal: 2; 9.9; 98.6; 986 mg/L
measured: 1. meaurement: 1.2; 9.9; 70; 738 mg/L; 2. measurement: 6.3; 12.5; 88; 951 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Round-bottom test tubes
- Material, size, headspace, fill volume: glass, calibrated to 10 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Adjustment of pH: not adjusted
- Photoperiod: not applicable
- Light intensity: no light during test


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation after 24 and 48 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not mentioned
- Range finding study: not mentioned

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 124 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

81 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

15 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

124 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid: yes, historical control (not tested in parallel to the test substance) (8.10.1996)
- Concentrations: 2 mg/L potassium dichromate
- Results: 100 % immobilised daphnia after 24 h

Reported statistics and error estimates:

no statistics performed, the EC50 values were calculated graphically (dose-response relationship).

Table #1: Effect of Marlowet 4753 on immobilisation of Daphnia magna

concentration [mg/L]	24 hour values			48 hour values
	number		%	number		%
	mobile	immobile	immobile	mobile	immobile	immobile
0 (control)	20	0	0	20	0	0
15	20	0	0	18	2	10
26	19	1	5	12	8	40
44	19	1	5	15	5	25
73	15	5	25	10	10	50
124	16	4	20	8	12	60

Validity criteria fulfilled:

yes

Executive summary:

The acute toxicity of Marlowet 4753 (Coconut oil, reaction products with polyethylene glycol and trimethylolpropane) to Daphnia magna was investigated in a static test according to 92/69/EEC.

After exposure for 48 h, the highest concentration at which no immobilization (<= 10 %) occurred was 15 mg/L, the LC100 was > 124 mg/L (highest concentration), the EC50 81 mg/L. The testsubstance is due to its properties insoluble in water. The concentrations employed were in all cases emulsions.

Description of key information

The acute toxicity of Marlowet 4753 (Coconut oil, reaction products with polyethylene glycol and trimethylolpropane) to Daphnia magna was investigated in a static test according to 92/69/EEC.

After exposure for 48 h, the highest concentration at which no immobilization (<= 10 %) occurred was 15 mg/L, the LC100 was > 124 mg/L (highest concentration), the EC50 81 mg/L. The testsubstance is due to its properties insoluble in water. The concentrations employed were in all cases emulsions.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

81 mg/L