Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

LL50 (96 h) > 100 mg/L (nominal)(Danio rerio, EU Method C.1)

Key value for chemical safety assessment

Additional information

The acute toxicity of Glycerides, C8-21 and C8-21-unsatd., mono- and di-, acetates (CAS No. 97593-30-1) to fish species was investigated in two studies (Weyers, 2007 and Richter, 2012). Both tests were conducted according to EU Method C.1 (Acute Toxicity for Fish), under GLP conditions under a static and semi-static water regime respectively. In the first test (Weyers, 2007), Danio rerio was exposed to the test substance for 96 h at 22 ± 1°C and the test concentration (limit test, 100 mg/L loading rate) measured at t=0 h, being below the limit of detection (LOD) of the GC method (< 0.062 mg/L). No effects were observed in test organisms after 96 hours (LL50 (96 h) > 100 mg/L (nominal)). A second study (Richter, 2012) was performed exposing Danio rerio to the test substance, this time under semi-static conditions. The test concentration (100 mg/L, loading rate) was measured by GC analysis. GC analysis of dissolved and undissolved test solution at 100 mg/L before the final filtration step resulted in test concentrations close to nominal values. During the testing period (performed with the filtered test solutions, without the undissolved fraction), measured concentrations remained below the limit of detection (LOD) of both GC (0.070 mg/L) and DOC (2 mg/L) methods. The water solubility of the substance was tested at 100 and 1000 mg/L, resulting in values of 3.5 and 21.9 mg/L, respectively. Nevertheless, differences in solubility of substances in pure water (water solubility test) and test medium (aquatic test) can be expected. Furthermore, considering that Glycerides, C8-21 and C8-21-unsatd., mono- and di-, acetates is expected to show high adsorption potential (log Koc values 3.7-4.2), most probably a significant fraction of test material was adsorbed to the filter. However, since the Water Accomodated Fraction (WAF) was performed, probably the adsorbed fraction of the substance also corresponded to the undissolved test material, and only the water soluble fraction was present in the test solution. Once again, no adverse effects were observed at the concentration tested and the LL50 (96 h) was determined to be > 100 mg/L (nominal loading rate).

In conclusion, no effects were observed up to the maximum achievable concentration in test medium.