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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the toxic effect of test chemical to Poecilia reticulata. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test solution was prepared by dissolving 1g of the test substance in 10 liters of deionised water with continuous stirring.
Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM
- Common name: Guppy fish
- Length at study initiation (length definition, mean, range and SD): average 2.5- 3.0 cm
- Weight at study initiation (mean and range, SD): average 0.275g

-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12-hour darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Test temperature:
24 °C
pH:
7-8
Dissolved oxygen:
7.4
Conductivity:
7.4
Nominal and measured concentrations:
Nomial Concentration :100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass bowl aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness

Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: other details not known
Sublethal observations / clinical signs:

Mortality and symptoms:

Nominal conc. (mg/l)

Number of fish

Mortality (cumulated) after

24h

48h

72h

96h

0 (control)

8

0

0

0

0

100

8

0

0

0

0

pH and oxygen content:

Nominal conc. (mg/l)

pH readings after

24h

48h

72h

96h

0 (control)

7.7

7.5

7.2

6.9

100

7.5

7.2

7.0

6.8

 

Nominal conc. (mg/l)

Oxygen content (mg/l) after

24h

48h

72h

96h

0 (control)

7.7

7.5

8.3

7.8

100

7.4

7.1

7.9

7.6

 

Test temperature

Nominal conc. (mg/l)

Temperature (°C) after

24h

48h

72h

96h

0 (control)

24

24

24

24

100

24

24

24

24

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experiment conducted at limit test i.e., 100 mg/L. the median lethal Concentrations [LC-50 (96 h)] for test chemical to guppy fish was determine to be >100 mg/L.
Executive summary:

Study was conducted to access the toxic effect of test chemical to fish Poecilia reticulata. Test was conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). Test was conducted in the static method, in which test chemical is not renewed, and experiment was run till 96 hours.

Limit test was performed, 100 mg/ L of test chemical was exposed to the test organisms and observed for any mortality for the period of 96 hours. According to the data obtained from the experiment there was no mortality in treated group, median lethal concentration of test chemical was determined to be >100 mg/L. According to CLP classification, the test chemical cannot be classified.

Description of key information

Study was conducted to access the toxic effect of test chemical to fish Poecilia reticulata. Test was conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). Test was conducted in the static method, in which test chemical is not renewed, and experiment was run till 96 hours.

Limit test was performed, 100 mg/ L of test chemical was exposed to the test organisms and observed for any mortality for the period of 96 hours. Based on the data obtained from the experiment there was no mortality in treated group, median lethal concentration of test chemical was determined to be >100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

Study was conducted to access the toxic effect of test chemical to fish Poecilia reticulata. Test was conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). Test was conducted in the static method, in which test chemical is not renewed, and experiment was run till 96 hours.

Limit test was performed, 100 mg/ L of test chemical was exposed to the test organisms and observed for any mortality for the period of 96 hours. Based on the data obtained from the experiment there was no mortality in treated group, median lethal concentration of test chemical was determined to be >100 mg/L. According to CLP classification, the test chemical cannot be classified.