Benzenamine, reaction products with aniline hydrochloride and nitrobenzene, hydrochlorides

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test method according to OECD TG 301 C. No data on GLP. .

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 301 C(Ready Biodegradability: Modified MITI Test (I))

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

not specified

Test organisms (species):

activated sludge

Details on inoculum:

- Initial biomass concentration: 30 mg/L

Test type:

other: Readily biodegradability

Water media type:

freshwater

Limit test:

no

Total exposure duration:

28 d

Details on test conditions:

TEST SYSTEM
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): BOD and test item concentration by HPLC.

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L.

Reference substance (positive control):

not specified

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: % biodegradability

The test item was determined not to be toxic in the biodegradability test although not been readily biodegradable.

Validity criteria fulfilled:

not specified

Conclusions:

The NOEC for microorganism toxicity of the test item was determined to be 100 mg/L.

Executive summary:

A readily biodegradation test was performed on 2 -methylcyclohexyl acetate by MITI according to OECD Guideline 301 C. 30 mg/L of activated sludge were exposed to 100 mg/L test item for 4 weeks. The biodegradation obtained by indirect analysis was 0 (basis for effect: BOD), and by direct analysis 2% (basis for effect: HPLC). The test item was deterined not to be toxic under test conditions. According to the current guidelines, the NOEC for microorganism toxicity was determined to be 100 mg/L.

Description of key information

Key study: OECD 301C. No data on GLP. The NOEC for microorganism toxicity was determined to be 100 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

100 mg/L

Additional information

Key study: Experimental results:

A readily biodegradation test was performed on test item by MITI according to OECD Guideline 301 C. The NOEC for microorganism toxicity was determined to be 100 mg/L.