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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
according to guideline
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
Version / remarks:
GLP compliance:
not specified
Details on sampling:
- Sampling intervals/frequency for test organisms: every two weeks, n=2, control fish analysis: Before the initiation and the termination of exposure, n=2
- Sampling intervals/frequency for test medium samples: Twice a week, n=1
- Recovery and blank test: Known amount of the test substance was added into test water and fish homogenate, respectively, then analyzed according to the procedures which eas established previously. Blank test was also performed in exactly same way without the test substance. These tests were carried out in duplicate. Recovery rates were used as correction factors for the determinaton of concentration of the test substance in analyzing samples.
Details on preparation of test solutions, spiked fish food or sediment:
It is necessary to prepare a solution of the test substance 100 times highly concentrated than that in the aquarium. If the test compound is not soluble enough in water, suitable solubilisers was used. More detailled informations are not available for each substance .
Test organisms (species):
Cyprinus carpio
Details on test organisms:
Supplier: Sugishima fish farm, Japan
Weight: about 30g, Length: about 10 cm, Lipid content: 2-6 % (at the initiation of exposure)
Route of exposure:
Test type:
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
8 wk
Test temperature:
Dissolved oxygen:
6-8 mg/L
Details on test conditions:
Test tank: 100L Glass tank
Number of fish: 15-20 fish/Level
Flow rate: 200-800 mL/min
Nominal and measured concentrations:
Considering of the result of preliminary test for 48-hr LC50 and analytically detecting limit of the test substance, test concentrations were decided.
Details on estimation of bioconcentration:
BCF = concentration of test substance in fish/concentration of test substance in water
Lipid content:
> 2 - < 6 %
Time point:
start of exposure
26 - 59
Remarks on result:
other: environment / dose:50 µg/L
37 - 65
Remarks on result:
other: environment / dose:5 µg/L
The bioconcentration factor of 1,2 -dichloro-4 -nitrobenzene ranges between 26 and 65 using Cyprinus carpio as test organism (MITI, 1992).
Executive summary:

The bioconcentration factor of 1,2 -dichloro-4 -nitrobenzene ranges between 26 and 65 using Cyprinus Carpio as test organism (MITI, 1992).

Description of key information

The bioaccumulation of 1,2-dichloro-4-nitrobenzene was tested using Cyprinus carpio as test organism after an exposure duration of 8 weeks in a flow-through system. Two concentrations were tested (5 µg/L and 50 µg/L). The BCF ranged between 25 and 65 (MITI, 1992).

Key value for chemical safety assessment

BCF (aquatic species):
65 dimensionless

Additional information