1,2-dichloro-4-nitrobenzene

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Principles of method if other than guideline:

Provisional procedure proposed by the Federal Environmental Agency for extended toxicology with Daphnia magna (01.01.1984)

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

- substance was dissolved in ethanol having a concentration of 100 µg/L
- solution was optical clear
- pH was not changes

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna strauss, strain IRCHA

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test temperature:

25 +/- 1 °C

Details on test conditions:

The test was performed under the following conditions:
- The test consists of 4 parallel test vessels (gastight) per concentration level and at least 4 for the control
- Test system: Each vessel was filled with 24 h-old Daphnia (1 organism/50 ml), the total number per concentration level was 20 organisms
- The parent animals in the control and test vessels were pipetted 3 times a week into freshly prepared test and control media.
- Dilution water: Source = Synthetic fresh water, Hardness = 2.5 mmol/L Ca + Mg, Na/K ratio = 10:1, pH = 8.0 +/- 0.1
- pH-values and oxygen concentration were measured during the test in two test-vessels per concentration level. The detected variation of these parameters had no negative influence on the organisms

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.025 mg/L

Nominal / measured:

nominal

Basis for effect:

reproduction

Tested concentration range: 0.0032 - 0.10 mg/L.
The author assumes the water solubility limit with 0.1 mg/L

Results of analytical monitoring
Nom conc. instant analysis analysis after 2 d
0.1 0.1 -0.12 0.09 - 0.1
0.05 0.05 - 0.06 0.04 - 0.05
0.025 0.020 - 0.030 0.020 - 0.026
0.012 0.010 - 0.02 0.010 - 0.012
Analysis after 2 d: In general, highest analytical values were obtained in controls. The lower values were measured in used Daphnia medium

Validity criteria fulfilled:

not applicable

Conclusions:

Long term toxicity to aquatic invertebrates test was done following the provisional procedure proposed by the Federal Environmental Agency for extended toxicology with Daphnia magna. Daphnia magna were exposed to test item for 21 days. The NOEC of 1,2 -dichloro-4 -nitrobenzene with respect to the reproduction rate of Daphnia magna accounts for 0.025 mg/L.

Executive summary:

Long term toxicity to aquatic invertebrates test was done following the provisional procedure proposed by the Federal Environmental Agency for extended toxicology with Daphnia magna. Daphnia magna were exposed to test item for 21 days. The NOEC of 1,2 -dichloro-4 -nitrobenzene with respect to the reproduction rate of Daphnia magna accounts for 0.025 mg/L.

Description of key information

Long term toxicity to aquatic invertebrates test was done following the provisional procedure proposed by the Federal Environmental Agency for extended toxicology with Daphnia magna. Daphnia magna were exposed to test item for 21 days. The NOEC of 1,2 -dichloro-4 -nitrobenzene with respect to the reproduction rate of Daphnia magna accounts for 0.025 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

NOEC

Remarks:

21days

Effect concentration:

0.025 mg/L