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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental result performed using standard methods of analysis
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
This study was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio) .Bowl aquaria containing 4 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material: Benzyl Propionate
- Molecular formula: C10H12O2
- Molecular weight: 164.2 g/mol
- Substance type: Organic
- Physical state: Colourless Liquid
-Smiles:c1(COC(CC)=O)ccccc1
-InChI:1S/C10H12O2/c1-2-10(11)12-8-9-6-4-3-5-7-9/h3-7H,2,8H2,1H3
-Purity:99.0%
-Batch No.:2014030601R-87
-Date of manufacturing:March, 2014

Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
The test substance was moderately soluble in water. Initially, stock solution was prepared by dissolving 1ml of the test substance in 1 liter of potable water (passed through reverse osmosis system) with 24 hrs of continuous stirring. From this stock solution, further test concentration was prepared for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish (Danio rerio)
- Source: Orange City Aquarium, Sitabuldi, Nagpur, MS (India)
- Age at study initiation: 3-4 months
- Length at study initiation : average 2.7 cm
- Weight at study initiation : 0.192 g (average)

ACCLIMATION
- Acclimation period: 15 days
- Acclimation conditions : same as test
- Type and amount of food during acclimation: Standard brand feed
- Feeding frequency during acclimation: once daily
- Health during acclimation (any mortality observed): No mortality
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
46.25
Test temperature:
23°C
pH:
6.58
Nominal and measured concentrations:
6.25,12.5,25,50,100mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: plastic aquaria
- Material, size, headspace, fill volume: 5liters
- Aeration: Aeration in test vessels was provided during the experiment after 24 hours of the start of the experiment
- No. of organisms per vessel: total=8
-Photoperiod :12 hours light and 12-hour darkness




Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
A 96 h study was conducted for CAS No. 122-63-4 on Zebra Fish (Danio rerio), at a test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria.
LC0 (96 hours) (highest loading at which no mortality was observed) = 12.5 mg/L
LC50 (96 hours) Experimental = > 12.5 mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 25 mg/L
Results with reference substance (positive control):
Not specified
Reported statistics and error estimates:
No data available
Sublethal observations / clinical signs:

Mortality and symptoms

NominalConcentration

(mg/l)

Number of fish

Mortality (cumulated) after

24h

48h

72h

96h

0 (control)

8

-

-

-

-

6.25

8

-

-

-

-

12.5

8

-

-

-

-

25

8

8

-

-

-

50

8

8

-

-

-

100

8

8

-

-

-

Visible Symptoms: Fishes were moving slowly as compared to control.

NominalConcentration(mg/l)

Number of fish

Symptoms after

24h

48h

72h

96h

0 (control)

8

NS8

NS8

NS8

NS8

6.25

8

M8

M8

M8

M8

12.5

8

M8

M8

M8

M8

25

8

-

-

-

-

50

8

-

-

-

-

100

8

-

-

-

-

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

pH and oxygen content:

NominalConcentration(mg/l)

pH readings after

24h

48h

72h

96h

0 (control)

7.8

7.8

7.8

7.8

6.25

7.9

7.8

7.9

7.9

12.5

7.9

7.9

7.9

7.9

25

-

-

-

-

50

-

-

-

-

100

-

-

-

-


Validity criteria fulfilled:
not specified
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.122-63-4 on ZEBRA FISH(Danio rerio)was found to be >12.5 mg/L.
Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Benzyl Propionate. The nominal concentration selected for the experiment were and test fish were exposed to 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L concentrations for 96 hours. The median lethal concentration (LC50) for Benzyl Propionate on Danio rerio in a 96 hours study on the basis of mortality effect was found to be >12.5 mg/L. Thus, on the basis

of this LC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded

that the substance, is classified under aquatic chronic 3 category

Benzyl Propionate (CAS No. 122 -63 -4) exhibits short term toxicityto fish.LC0 (96 hours) (highest loading at which no mortality was observed) = 12.5 mg/L

LC50 (96 hours) Experimental = >12.5 mg/L

This LC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water thus it can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria. 

Description of key information

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Benzyl Propionate. The nominal concentration selected for the experiment were and test fish were exposed to 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L concentrations for 96 hours. The median lethal concentration (LC50) for Benzyl Propionate on Danio rerio in a 96 hours study on the basis of mortality effect was found to be >12.5 mg/L. Thus, on the basis

of this LC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded

that the substance, is classified under aquatic chronic 3 category

Benzyl Propionate (CAS No. 122 -63 -4) exhibits short term toxicityto fish.LC0 (96 hours) (highest loading at which no mortality was observed) = 12.5 mg/L

LC50 (96 hours) Experimental = >12.5 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
12.5 mg/L

Additional information

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Benzyl Propionate. The nominal concentration selected for the experiment were and test fish were exposed to 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L concentrations for 96 hours. The median lethal concentration (LC50) for Benzyl Propionate on Danio rerio in a 96 hours study on the basis of mortality effect was found to be >12.5 mg/L. Thus, on the basis

of this LC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded

that the substance, is classified under aquatic chronic 3 category

Benzyl Propionate (CAS No. 122 -63 -4) exhibits short term toxicityto fish.LC0 (96 hours) (highest loading at which no mortality was observed) = 12.5 mg/L

LC50 (96 hours) Experimental = >12.5 mg/L

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