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Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23.01.1990 - 25.01.1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Remarks:
"Gute Laborparxis" 1983
Analytical monitoring:
no
Test organisms (species):
activated sludge
Details on inoculum:
- Laboratory culture: yes
- Name and location of sewage treatment plant where inoculum was collected: 10L from OECD lab, 19.01.1990
- Pretreatment: no
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Nominal and measured concentrations:
100, 180, 320, 560, 1000 mg/L
Reference substance (positive control):
yes
Remarks:
3,5-dichlorphenol
Duration:
3 h
Dose descriptor:
EC50
Remarks:
Respiration inhibition
Effect conc.:
914 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration

































Test item concentration (mg/L)Respiration rate (mg/L*h)Inhibition (%)

100


247

180


247

320


247

560


1831

1000


1060
Validity criteria fulfilled:
yes
Remarks:
The validity of the test was checked by means of a graphical evaluation of the reference substance.
Conclusions:
The EC50(3h) on microorganisms for the test substance was 914 mg/L (nominal).
Executive summary:

The test item was assessed in an test on toxicity to bacteria with 3,5-Xylidine according to ISO 8192-1986 (E), similar to OECD 209 and following GLP. Activated sludge was incubated for 3h to nominal test concentrations of 100, 180, 320, 560, 1000 mg/L- The respration rate was measured. The EC50(3h) on microorganisms for the test substance was 914 mg/L (nominal).

Description of key information

ISO 8192-1986 (E), similar to OECD 209, activated sludge, GLP, nominal test concentrations of 100, 180, 320, 560, 1000 mg/L- The EC50(3h) 914 mg/L (nominal).

Key value for chemical safety assessment

EC50 for microorganisms:
914 mg/L

Additional information