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Short term tests with freshwater species of three trophic levels (fish, daphnia, algae) are available for Glycerides, C8-18 and C18-unsatd. mono- and di-, acetates (CAS No. 91052 -13 -0). Fish and daphnia did not show any effects up to the limit of the water solubility, but for aquatic algae (Pseudokirchneriella subcapitata) an ErC50 of 11 mg/L and a NOEC of 6.6 mg/L was determined. Long-term tests with fish and aquatic invertebrates were not conducted as the chemical safety assessment does not indicate a need for further long-term investigations.The inhibitory effects on the respiration of activated sludge of Glycerides, C8-18 and C18-unsatd. mono- and di-, acetates were not investigated in a separate test, as a toxicity control in the ready biodegradability test showed that the test substance is not inhibiting the respiration of sewage treatment microorganisms at a concentration of 15.1 mg/L. Thus, Glycerides, C8-18 and C18-unsatd. mono- and di-, acetates are of low concern for sewage treatment plants, but show low toxic effects on aquatic algae.