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EC number: 300-326-6 | CAS number: 93925-25-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Between 15 March 1974 and 18 April 1974
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Valid and conclusive pre-GLP study comparable to guideline, basic data reported
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- 1987
- Deviations:
- yes
- Remarks:
- Necropsy omitted, administered volume in excess of guideline recommendations
- GLP compliance:
- no
- Remarks:
- The experiment was performed before the first GLP standard was defined by FDA in 1976, therefore no formal GLP was possible. Nonetheless it can be assumed that comparable standards applied as the test was conducted in a specialized laboratory.
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Phosphonic acid, mixed C12-20-alkyl and C14-18-unsatd. alkyl derivs.
- EC Number:
- 300-326-6
- EC Name:
- Phosphonic acid, mixed C12-20-alkyl and C14-18-unsatd. alkyl derivs.
- Cas Number:
- 93925-25-8
- Molecular formula:
- Complex
- IUPAC Name:
- Phosphonic acid, mixed C12-20-alkyl and C14-18-unsatd. alkyl derivs.
- Test material form:
- liquid: viscous
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Sherman-Wistar (albino)
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: Animals weighed between 200 and 250 g.
- Fasting period before study: Test animals were fasted overnight (24 h) before dose administration.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Doses:
- 2, 4, 8, 16, or 32 mL/kg; given the submission item relative density value 910 kg/m³, the doses correspond to 1820, 3640, 7280, 14,560 and 29,120 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 d
- Frequency of observations and weighing: Daily observation, weighing at study initiation only
- Necropsy of survivors performed: No
- Other examinations performed: The animals were observed for 14 d for signs of toxicity and mortality.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 32 mL/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: Mortality: 1/5 and 2/5 at 16 and 32 mL/kg, respectively
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 29 120 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: Converted using the density value 910 kg/m³
- Sex:
- male
- Dose descriptor:
- LD0
- Effect level:
- >= 7 280 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: No difference to control
- Mortality:
- At 16 mL/kg bw one test animal was found dead on day 2.
At 32 mL/kg bw one test animal was found dead on day 1 and a second one on day 2. - Clinical signs:
- other: No observations
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Remarks:
- Migrated information
- Conclusions:
- Not acute toxic, LD0 ≥ 8 mL/kg bw or ≥ 7280 mg/kg bw and LD 50 > 32 mL/kg bw or > 29,120 mg/kg bw
- Executive summary:
The acute toxicity of the test item by oral route was investigated in a pre-GLP study using male albino Sherman-Wistar rats according to a protocol comparable to the OECD TG 401 (1987) standards. At variance to the guideline no necropsy was performed and the test item was applied in a volume exceeding 1 mL per 100 g bw. The experiment was performed before the first GLP standard was defined by FDA in 1976, therefore no formal GLP was possible. Nonetheless it can be assumed that comparable standards applied as the test was conducted in a specialized laboratory. The reporting is limited but sufficient information is given. The experiment is deemed valid, conclusive and thus suitable for assessment with minor restrictions.
Groups of test animals, each consisting of 5 individuals weighing between 200 and 250 g were fasted overnight and administered 2, 4, 8, 16 or 32 mL/kg by stomach tube, which corresponds to 1820, 3640, 7280, 14,560 and 29,120 mg/kg bw. The animals were observed for 14 d post exposure for signs of toxicity and mortality.
Mortality was observed at 16 and 32 mL/kg, where 1 and 2 animals died, respectively, until day 2. No observation of sublethal effects was made. Therefore the LD50 is > 32 mL/kg bw or > 29,120 mg/kg bw. At 8 mL/kg bw or 7280 mg/kg bw no mortality occurred, which means that the LD0 (discriminating dose) can be assigned to be greater or equal to this level.
In summary no effects were observed in the classifiable range up to 2000 mg/kg bw (CLP) or even 5000 mg/kg bw (GHS). Therefore, on the basis of this study, classification is not required for the test item in accordance with EU CLP Regulation (EC) No 1272/2008 and the absence of acute oral toxicity is evidenced.
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