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Diss Factsheets

Administrative data

Description of key information

oral: LD50 > 2000 < 5000 mg/kg bw (WoE: Rudnev et al. 1979, rat and mouse, Smyth et al. 1954, rat)
inhalation: LC 50 (rat) > 7.74 mg/L air (90-0524-FGT)

Key value for chemical safety assessment

Acute toxicity: via oral route

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed

Acute toxicity: via inhalation route

Link to relevant study records
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March 1st- 31th, 1990
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP - Guideline study
according to guideline
OECD Guideline 403 (Acute Inhalation Toxicity)
Version / remarks:
, 1981
GLP compliance:
Test type:
standard acute method
Limit test:
Details on test animals or test system and environmental conditions:
- Source: Lippische Versuchstierzucht HAGEMANN GmbH & Co., Exertal 1, Germany
- Age at study initiation: 51 - 54 days
- Weight at study initiation: 229 - 249 g (males); 212 - 250 g (females)
- Fasting period before study: 16 hours
- Housing: in groups of two or three in MAKROLON cages (type III)
- Diet: ad libitum standardized diet for rats ALTROMIN 1324 (ALTROMIN GmbH, Lage/Lippe, Germany); analysed for contaminants
- Water: ad libitum tap water; analysed regularly
- Acclimation period: at least 5 days

- Temperature (°C): 21 +/-2
- Humidity (%): 50 +/- 10
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: From: Feb. 1990 To: March 15th, 1990
Route of administration:
inhalation: aerosol
Type of inhalation exposure:
nose only
Details on inhalation exposure:
- Exposure apparatus: dynamic inhalation apparatus with a nose-only exposure according to Kimmerle & Trepper.
- Exposure chamber volume: 40 L
- Method of holding animals in test chamber: Apparatus consists of a cylindrical exposure chamber which holds a maximum of 20 animals in pyrex tubes at the edge of the chamber in a radial position
- Source and rate of air: 400 L/h
- System of generating particulates/aerosols: A mixture of test substance and air was obtained using a spray-jet. The spray-jet was fed with compressed air from a compressor and with the test article using an infusion pump and a 50 mL syringe.
- Temperature, humidity, pressure in air chamber: 22 +/- 3°C

- Brief description of analytical method used: GC-analyses of two air samples
- Samples taken from breathing zone: yes

TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: Test substance is of volatile nature at room temperture (vapour pressure 10700 Pa at 20°C). This resulted in an almost complete gas phase in the inhalation chamber after the test substance air mixture escaped from the spray-jet.

Analytical verification of test atmosphere concentrations:
Duration of exposure:
4 h
1.49 and 7.74 mg/L air (actual concentrations)
No. of animals per sex per dose:
Control animals:
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
Observations and clinical examiniations: on the day of exposure 5, 15, 30 and 60 min, 3 h and 24 h after end of exposure; during recovery period at least once a day until all symptoms had subsided, thereafter each working day.
Individual body weight: before the exposure and after exposure in weekly intervals; if animals died body weight at time-point of death was recorded.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight gain
Dose descriptor:
Effect level:
> 7.74 mg/L air
Based on:
test mat.
Exp. duration:
4 h
No mortality occured.
Clinical signs:
other: No substance-related intolerance reactions were observed.
Body weight:
No inhibition of body weight gain was examined.
Gross pathology:
Macroscopic inspection revealed no pathological findings.
Interpretation of results:
study cannot be used for classification
Migrated information
Endpoint conclusion
Endpoint conclusion:
no adverse effect observed

Acute toxicity: via dermal route

Endpoint conclusion
Endpoint conclusion:
no study available

Additional information


In acute oral toxicity tests Rudnev et al. (1979) exposed albino rats and white mice to 1-chlorobutane. LD50s of 2200 mg/kg bw and 5600 mg/kg bw were examined, respectively, indicating that rats may be more sensitive to 1-chlorobutane than mice. No further information on mortality, clinical signs or necropsy were provided. In another acute oral toxicity test 5 female rats were intubated with a logarithmic series of doses (Smyth et al., 1954). 14 days after dosing, mortality was considered to be complete (LD50: 2670 mg/kg bw).

Taken together in a weight of evidence approach, the data indicate that the test material has a low toxic potential to rats and mice after oral ingestion.



In a GLP-guideline study according to OECD 403 (1981) rats were exposed to 1-chlorobutane via aerosol inhalation to actual concentrations of 1.49 and 7.74 mg/L air over an exposure period of 4 hours (90-0524-FGT). No substance-related toxicity was observed and no mortality occurred. Macroscopic inspection revealed no pathological findings. Thus the LC50 was determined to be > 7.74 mg/L air. An inhalative LC50 value for male rats was reported as > 8000 ppm (> 30 mg/L) after an exposure period of 4 hours to the vapour of 1-chlorobutane by Smyth et al.(1954). Two of six male rats died within an observation period of 14 days after exposure.



In an acute dermal toxicity study with rabbits, available as short abstract only, no effects were detected after 24 hours of exposure to very high amounts of 1-chlorobutane (LD50 > 20 ml/kg).The test substance was applied occlusively to 1/10 of the body surface (Smyth et al., 1954).

Justification for selection of acute toxicity – oral endpoint
Weight of evidence approach.

Justification for classification or non-classification

The available data is conclusive but not sufficient for classification according to DSD (67/548/EEC) and CLP (1272/2008/EC).