1,2-epoxybutane

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: basic data given, comparable to guideline/standards

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Principles of method if other than guideline:

dynamic inhalation method with analytical and nominal determination of the concentration.

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Details on test material:

purity: 99.9%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Weight of the animals: 185 +/- 15 g. Standard diet and tap water ad libitum during the observation period.

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

ANALYTICAL EXAMINATIONS:
1. Apparatus, chemicals and solutions:
- gaschromatograph HP 5840 A
- dimethylformamid
2. Sampling:
- apparatus: 2 consecutive connected absorption vessels and a downstream fritted bottle
- sorption agent: dimethylformamide
- sampling rate: 1 liter/min
- sampling amount: 3 liter
- sampling place: near the animals' nose
- sampling probe: diameter 4 mm
3. Analytical determination method:
- gaschromatographically
- principle of the method: n-butylenoxide was determined in dimethylformamide

Supplying of the test substance with a prolonged infusion pump in a constant volume on a 93°C heated evaporator; the refined vapours were mixed with a fresh air flow and were conducted through an inhalation chamber with a chamber content of about 200 liters.

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

6.3 mg/l

No. of animals per sex per dose:

10

Control animals:

yes

Details on study design:

Dynamic inhalation test with analytical monitoring; post exposure observation period: 14 days
As a first orientating dose, a concentration was used which allow a classification in a toxic class. A dose level ought to allow an no effect level (no mortality).
The inhalation mixture was offered for 4 hours.
The body weight of the animals were checked before starting of the study, after 7 days and at the end of the observation period. Clincal signs and mortality were checked daily.
After the 14-day observation period the animals were killed and a pathological-anatomical evaluation was done.

Statistics:

none

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 6.3 mg/L air (analytical)

Exp. duration:

4 h

Remarks on result:

other: No mortalities.

Effect levels 2

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 25 mg/L air (analytical)

Exp. duration:

1 h

Remarks on result:

other: No mortalities.

Mortality:

none

Clinical signs:

other: no symptoms

Gross pathology:

nothing abnormal detected

Any other information on results incl. tables

Absolute body weight growth:

Mean body weight	before starting		after 7 days		after 14 days
	males	females	males	females	males	females
Group 1
Body weight in g	185	178	219	190	258	206
No. of animals	10	10	10	10	10	10
Control
Body weight in g	178	180	219	190	254	210
No. of animals	10	10	10	10	10	10

Relative body weight growth:

Mean body weight	before starting		after 7 days		after 14 days
	males	females	males	females	males	females
Group 1
Body weight in g	0	0	34	12	73	28
No. of animals	10	10	10	10	10	10
Control
Body weight in g	0	0	41	10	76	30
No. of animals	10	10	10	10	10	10

No significant differences regarding body weight growth in comparison to the air control group.