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EC number: 203-940-1 | CAS number: 112-15-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 Jul - 20 Sep 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Circular on Test Methods of New Chemical Substances (Japan), Biodegradation test
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: mixture of activated sludge from STP with freshwater from rivers and lakes, marine water and marine sediment
- Details on inoculum:
- - Source of inoculum/activated sludge (location, sampling depth): location: 10 sites (4 sewage plants, 3 rivers, 1 lake and 2 bays) around Japan, sampling depth: return sludge in sewage plants, surface water and surface soil of foreshore
- Laboratory culture: Thirty minutes after stopping of aeration, discard about one third of the whole volume of supernatant and add an equal volume of a solution (pH adapted to 7.0 with NaOH) containing 0.1% each of glucose, peptone and potassium orthophosphate, to the settled material and re-commence aeration. This procedure was repeated once per day.
- Temperature: 25 ± 2 °C
- Storage conditions: observed appearance and general condition of sludge, determined sediments, pH, temperature and dissolved oxygen and observed with a light microscope, whether it is normal.
- Storage length: started to use: 16 Jul 1991
- Preparation of inoculum for exposure: Activated sludge from STP mixed with
- Concentration of sludge: 4600 mg/L
- Water filtered: no - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: recommended medium in the guideline
- Test temperature: 25 ± 1 °C
- Suspended solids concentration: 30 mg/L
- pH: 5.6 - 7.2
TEST SYSTEM
- Culturing apparatus: closed oxygen consumption measuring machine with 300 mL culture bottle (coulometer)
- Number of culture flasks/concentration: 1 (water + test substance), 3 (sludge + test sub.), 1 (sludge + reference sub.), 1 (sludge)
- Measuring equipment: Coulometer
- Details of trap for CO2 and volatile organics if used: soda lime
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes - Reference substance:
- aniline
- Remarks:
- 100 mg/L
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 101
- Sampling time:
- 28 d
- Details on results:
- No 10-day window required. Substance was readily biodegradable.
- Results with reference substance:
- The oxygen consumption for aniline in the above used test system reached 68% and 90% degradation after 7 days and 14 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Complete biodegradation was observed after 28 days. Since the substance reached the pass level of > 60% after 28 days (no 10-day window required for OECD 301C), 2-(2-ethoxyethoxy)ethyl acetate is regarded as being readily biodegradable according to the OECD criteria.
- Executive summary:
Ready biodegradability has been investigated in a study performed according to OECD TG 301C employing a mixture of activated sludge from STP with freshwater from rivers and lakes, marine water and marine sediment as inoculum. Complete biodegradation was observed after 28 days. Since the substance reached the pass level of > 60% after 28 days (no 10-day window required for OECD 301C), 2-(2-ethoxyethoxy)ethyl acetate is regarded as being readily biodegradable according to the OECD criteria.
Reference
Table 1. Results of analysis for BOD, DOC and GC
|
water + test sub. |
sludge + test sub. |
Theoretical Amount |
|||
sample No. 4 |
sample No. 1 |
sample No. 2 |
sample No. 3 |
|||
BOD (*1) |
mg |
0.0 |
53.8 |
57.0 |
56.0 |
55.1 |
DOC (residue of test substance) |
mg |
16.5 |
0.9 |
0.8 |
0.8 |
16.5 |
% (*2) |
100 |
5 |
5 |
5 |
||
GC |
mg |
28.6 |
0 |
0 |
0 |
30.3 |
% (*2) |
94 |
0 |
0 |
0 |
(*1) (sludge + test sub.) was calculated by substracting blank values.
(*2) Residue and production percentage was calculated as followed.
Residue percentage = Residue amount (mg) / Theoretical amount (mg) x 100
Table 2. Chart of BOD
|
BOD (mg) |
||
|
7th day |
14th day |
28th day |
Sludge + Test substance |
27.2 |
52.8 |
60.0 |
Sludge + Test substance |
26.8 |
57.8 |
63.2 |
Sludge + Test substance |
34.0 |
58.5 |
62.2 |
Water + Test substance |
0.0 |
0.0 |
0.0 |
Control blank |
1.5 |
3.5 |
6.2 |
Sludge + Aniline |
63.2 |
84.5 |
91.8 |
Description of key information
Readily biodegradable: 101% (BOD) after 28 d (OECD 301C)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
One experimental study supported by handbook data is available investigating the ready biodegradability of 2-(2-ethoxyethoxy)ethyl acetate (CAS 112-15-2). The key study was performed according to OECD 301C (GLP) with a mixture of activated sludge from STP with freshwater from rivers and lakes, marine water and marine sediment as inoculum (Ministry of Economy, Trade and Industry, Japan, 1991). A mean biodegradation of 101% was observed after 28 d. Since the substance reached the pass level of > 60% after 28 d (no 10-day window required for OECD 301C), 2-(2-ethoxyethoxy)ethyl acetate is stated to be readily biodegradable according to the OECD criteria. Further handbook data indicate a biodegradation of 90.1% (O2 consumption) after 20 d (Verschueren, 1996) for this substance. Since no guideline is mentioned and no degradation kinetics are available, this result cannot be used for an assessment of ready biodegradability of the test substance. Nevertheless, it supports the result from the key study and therefore, extensive biodegradation of 2-(2-ethoxyethoxy)ethyl acetate can be assumed . In conclusion, 2-(2-ethoxyethoxy)ethyl acetate can be classified as readily biodegradable according to the OECD criteria.
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