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EC number: 204-798-3 | CAS number: 126-71-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Triisobutyl phosphate
- EC Number:
- 204-798-3
- EC Name:
- Triisobutyl phosphate
- Cas Number:
- 126-71-6
- Molecular formula:
- C12H27O4P
- IUPAC Name:
- triisobutyl phosphate
Constituent 1
- Specific details on test material used for the study:
- - Name of test material: Triisobutylphosphat
- Physical state: liquid
- Storage condition of test material: at 20°C in the dark
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: HOECHST AG, Kastengrund, SPF-Zucht
- Age at study initiation: 8-10 weeks
- Weight at study initiation: male; 201-211g. females; 182-193g
- Fasting period before study: no data
- Housing: Makrolon cages (type 4) on Softwood pellets
- Diet (ad libitum): Altromin 1324 (Altromin-GmbH, Lage/Lippe)
- Water (ad libitum): tap water in plastic bottles
- Acclimation period: minimal 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2 °C
- Humidity (%): 50 ± 20 %
- Air changes (per hr): not specified
- Photoperiod (hrs dark / hrs light): 12 hours
IN-LIFE DATES: not specified
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: not specified
- Exposure chamber volume: 60 Liter
- Method of holding animals in test chamber: not specified
- Source and rate of air: 800 L/hr
- System of generating particulates/aerosols: the test substance was injected by static speed by a continuous infusion apparatus into the nozzle. Through riser the aerosol was injected into the inhalation chamber.
- Method of particle size determination: The cascade impactor was operated with a vacuum throughput of 9.5L/Min, resulting in a flow rate of ca. 1.25 m/sec The calculation of the mean aerodynamic diameter and geometric standard deviation of the particle size distribution was determined by probit analysis
(Probit against logarithms of the cumulative frequency of the aerodynamic diameter)
- Treatment of exhaust air: Bottom of the inhalation chamber was a suction device, with Buehler filters, a wash bottle with Tetrachloroethylene, a wash bottle with deionized water and a Calciumchlorid bottle where air was extracted with 1100 Liters of air per hour.
- Temperature, humidity, pressure in air chamber: a with water 1000 mL bottle flows through with 100 liters of air per hour. The with moisture-enriched air was passed directly into the chamber.
TEST ATMOSPHERE
- Brief description of analytical method used: during inhalation, the test amosphere was continuous tested by Hartmann Brown; CO, CO2, 02, humidity and temperature in the exposure chamber is measured.
- Samples taken from breathing zone: yes
VEHICLE
- Concentration of test material in vehicle: 5.14mg/L
- Justification of choice of vehicle: not specified
TEST ATMOSPHERE
- Particle size distribution:
< 0,6 µm: 3,9 %
0,6 - 0,8 µm: 17,6 %
0,8 - 1,5 µm: 36,3 %
1,5 - 3,0 µm: 39,2 %
3,0 - 4,8 µm: 2,9 %
4,8 - 7,0 µm: 0 %
7,0 - 10,3 µm: 0 %
>10,3 µm: 0 %
cumulative: 99,9%
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): Anderson-Kaskadenimpaktor Mark III, Firma Anderson Samples INC, Atlanta
The analysis of particle size distribution showed a mean mass-based aerodynamic diameter (MMAD) of 1.3 microns with a geometric standard deviation of 1.6.
CLASS METHOD (if applicable)
- Rationale for the selection of the starting concentration: - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 5.14 mg/liter
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 21 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology - Statistics:
- Not specified
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 5.14 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- other: no mortality occurred
- Mortality:
- No mortality occurred
- Clinical signs:
- other: The animals showed irregular breathing, jerky breathing, gasping, cooing and crackling sound of breathing, tremors, long-legged, uncoordinated and staggering gait, decreased spontaneous activity, ruffled fur, squatting, retracted flanks, reduced parking a
- Body weight:
- In the first week some effect in body weight were observed. At the end of the observation period all animals showed normal body weight gain, except for one female.
- Gross pathology:
- Two male and found females showed effects on lungs. The other animals were free of macroscopic effects.
Applicant's summary and conclusion
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