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Environmental fate & pathways

Biodegradation in soil

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Administrative data

Endpoint:
biodegradation in soil
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication which meets basic scientific principles.

Data source

Reference
Title:
No information
Author:
Graveel JG, Sommers LE, Nelson W (1985). Sites of benzidine, alpha-naphthylamine and p-toluidine retention in soils. Environ. Toxicol. Chem. 4, 607-613.

Materials and methods

Principles of method if other than guideline:
radiotracer experiment
GLP compliance:
not specified
Test type:
laboratory

Test material

Constituent 1
Chemical structure
Reference substance name:
p-toluidine
EC Number:
203-403-1
EC Name:
p-toluidine
Cas Number:
106-49-0
Molecular formula:
C7H9N
IUPAC Name:
p-toluidine
Details on test material:
- Name of test material (as cited in study report): p-Toluidine
- labeled p-toluidine: uniformly 14C-ring-labeled
- unlabeled p-toluidine: Sigma Technical Grade; initial concentration was 0.19 M


Radiolabelling:
yes

Results and discussion

Half-life / dissipation time of parent compound
Type:
(pseudo-)first order (= half-life)
Details on results:
Extracted amines at day zero:
a) EtAc-MeOH: 67%
b) NH4OAc: 12.3%
c)NaOH: 8.4%
Sum: 87.7%

Extracted amines after 63 d incubation period:
a) EtAc-MeOH: 13.0% +/- 4.5%
b) H4OAc: 3.1% +/-0.4%
c) NaOH: 31.8% +/-2.5%
Sum: 47.9%

Decomposition of p-toluidine was low (ca. 15% CO2 evolution of added conc.)

Any other information on results incl. tables

The extraction at day zero indicates that p-toluidine was initially loosely associated with clay and organic matter through electrostatic interactions, hydrophobic bonding or reversible amine linkages with humate carbonyls (reported soil pH of approx. 5 resulted in protonation of p-toluidine). The increase of p-toluidine in the NaOH fraction until day 63 suggests that the amine is increasingly covalently linked to humic components in soil.

Applicant's summary and conclusion