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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given

Data source

Reference
Title:
No information
Author:
Nalecz-Jawecki G, Sawicki J (1999). SPIROTOX - A new tool for testing the toxicity of volatile compounds. Chemosphere 38 (14), 3211-3218.

Materials and methods

Principles of method if other than guideline:
Spirotox test: Toxicity of volatile compounds to the protozoa Spirostomum ambiguum was tested in polysterene multiwell plates.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
p-toluidine
EC Number:
203-403-1
EC Name:
p-toluidine
Cas Number:
106-49-0
Molecular formula:
C7H9N
IUPAC Name:
p-toluidine
Details on test material:
- Name of test material (as cited in study report): p-toluidine
- Analytical purity: analytical purity

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
- stock dilutions were prepared in deionized water or, if a toxicant was insoluble in water, in methanol
- dilutions were prepared using Tyrod solution (see ¿any other information on materials..." => "Test organism")

Test organisms

Test organisms (species):
other: protozoa: Spirostomum ambiguum

Test conditions

Details on test conditions:
Type: aquatic

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
1.8 mmol/L
Nominal / measured:
nominal
Remarks on result:
other: SD ± 0.41
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.93 mmol/L
Nominal / measured:
nominal
Remarks on result:
other: SD ± 0.01
Duration:
24 h
Dose descriptor:
other: LC50
Effect conc.:
2.87 mmol/L
Nominal / measured:
nominal
Remarks on result:
other: SD ± 0.55
Duration:
48 h
Dose descriptor:
other: LC50
Effect conc.:
1.52 mmol/L
Nominal / measured:
nominal
Remarks on result:
other: SD ± 0.26

Any other information on results incl. tables

All results are expressed in mmol/l. The 24-h values correspond to:

=> 24-h EC50 = 192.9 mg/l

=> 24-h LC50 = 307.5 mg/

Applicant's summary and conclusion