Ferrate(1-), [[N,N'-1,2-ethanediylbis[N-[[2-(hydroxy-.kappa.O)phenyl]methyl]glycinato-.kappa.N,.kappa.O]](4-)]-, sodium (1:1), (OC-6-13)-

Endpoint summary

Additional information

Toxicity to fish

Oncorhynchus mykiss was used as test organism in an experiment conducted in accordance to OECD Guideline 203 / EU Method C.1 (Rzodeczko, 2008). The following results were obtained: LC0(96) >= 100 mg/L, LC50(96h) > 100 mg/L and LC100(96h) > 100 mg/L. Based on these results, which are used for the chemical safety assessment and in accordance to REACH Regulation (EC) 1907/2006 (Annex IX, Section 9.1.6, column 2), no further experimental investigation concerning toxicity to fish is triggered.

Toxicity to invertebrates

An experiment to investigate the toxicity of Fe(Na)HBED towards Daphnia magna was conducted in accordance to OECD Guideline 202 / EU Method C.2 (Nierzedska, 2008). The preliminary as well as the definitive test were conducted as limit test, with one single concentration (100 mg/L). No effects were observed, concluding that the EC0(48h) >= 100 mg/L, the EC50(48h) and EC100(48h) > 100 mg/L. Also for aquatic invertebrates no further toxicity experiment is triggered.

Toxicity to aquatic plants

The microphyte Pseudokirchneriella subcapitata was used in an experiment according to OECD Guideline 201 / EU Method C.3 (Nierzedska, 2009). Slight deviations from the applied test guidelines were recorded, since the mean coefficient of variation for section-by-section specific growth rates in the control cultures was exceeded (45.1 % instead 35 %) and the illumination was slightly greater (8910 - 9260 lux instead of 4400 - 8800 lux). Based on nominal concentrations, the following results are presented concerning specific growth rate: ErC10(72h): 34.40 mg/L, ErC20(72h): 56.66 mg/L and ErC50(72h) > 100 mg/L. Concerning yield inhibition (biomass) the following nominal results are obtained: EyC10(72h): 10.20 mg/L, EyC20(72h): 16.52 mg/L and EyC50(72h): 41.33 mg/L. Nominal LOEC and NOEC are < 10 mg/L and are calculated from yield and average specific growth rate. Based on the geometric mean from measured concentrations of the test substance, the results concerning specific growth rate are as followed: ErC10(72h): 26.78 mg/L, ErC20(72h): 42.54 mg/L and ErC50(72h) > 70.92 mg/L. Yield inhibition (biomass) resulted in the following results: EyC10(72h) < 9.75 mg/L, EyC20(72h): 14.55 mg/L and EyC50(72h): 32.22 mg/L. Measured LOEC and NOEC are < 9.75 mg/L, calculated from yield and average specific growth rate.

Toxicity to microorganisms

Fe(Na)HBED was not investigated experimentally for its toxicity towards aquatic microorganisms. A read-across approach is intended to the structural analogue Acetic acid, oxo-, sodium salt, reaction products with ethylenediamine and phenol, iron sodium salts (CAS 84539-55-9), wherefore experimental data according to OECD Guideline 209 is available (BASF SE, 2009). For further details of the read-across procedure, please refer to the separate statement by Chemservice S.A., 2012. The following results were obtained: EC10(3h): 450 mg/L, EC50(3h) > 1000 mg/L and EC80(3h) > 1000 mg/L.

Toxicity to other aquatic organisms

No further investigation for the aquatic compartment is triggered.