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EC number: 200-909-4 | CAS number: 75-86-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: - Basic data given: comparable to guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- Principles of method if other than guideline:
- Deviations from guideline: at least 5 animals of the same sex should be used at each dose level. 2 males and 2 females per group were used in this study.
- GLP compliance:
- no
- Remarks:
- Study performed before implementation of GLP
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2-hydroxy-2-methylpropionitrile
- EC Number:
- 200-909-4
- EC Name:
- 2-hydroxy-2-methylpropionitrile
- Cas Number:
- 75-86-5
- Molecular formula:
- C4H7NO
- IUPAC Name:
- 2-hydroxy-2-methylpropanenitrile
- Details on test material:
- - Name of test material (as cited in study report): acteone cyanohydrin, also known as methyl lactonitrile or alpha-hydroxyisobutyronitrile
- Physical state: light amber liquid
- Typical analysis for the test item: acetone cyanohydrin wt %: 98.0; acetone wt %: 0.80, water wt %: 0.40, hydrogen cyanide wt %: 0.35 %, stabilizer and inert material wt %: 0.20; in total: 99.75 %
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Langshaw Farms, Augusta, Michigan, U.S.A.
- Age at study initiation: no data
- Weight at study initiation: male: 3280 - 3530 g, female: 3200 - 3490 g
- Fasting period before study: no data
- Housing: individual holding cages with free access to food and water
- Diet: ad libitum, commercial laboratory chow (Ralston Purina Company, St. Louis, Missouri, U.S.A)
- Water: ad libitum
- Acclimation period: 2 weeks
ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12 h light and dark cycle
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- water
- Details on dermal exposure:
- TEST SITE
24 hours prior to application of the test item, the entire trunk of 2 male and 2 female rabbits/dose level was clipped free of hair with electric clipper.
- Area of exposure: trunk
- Type of wrap if used: heavy-gauge SARAN Film sleeve held in place with rubber bands. The plastic sleeve was covered by a cloth bandage taped securely to the marginal hair.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): skin was washed with a mild soap and water, rinsed thoroughly and dried with a soft disposable towel
- Time after start of exposure: after 24 h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): unstated
- Concentration (if solution): 6, 25, 40 mg/kg
- Constant volume or concentration used: unstated - Duration of exposure:
- 24 h
- Doses:
- 6, 25, 40 mg/kg of test item (10 % aq.)
- No. of animals per sex per dose:
- 2 males and 2 females/ dose level
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: frequently, after 24 h observations for erythema, edema, necrosis
- Frequency of weighing: before and after the 24 h exposure, and at 1 and 2 weeks post-treatment
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, - Statistics:
- The LD50 was calculated by the moving average method (Thompson and Weil, 1952). The approximate slope was calculated as 1/2 log10 (LD84/LD16) (Thompson, W.R. and C.S. Weil, Biometrics, Vol. 8, No. 1, March 1952, pp. 51-54
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 16 mg/kg bw
- 95% CL:
- >= 8 - <= 28
- Remarks on result:
- other: estimation
- Mortality:
- 6 mg/kg bw: 0/4 animals
25 mg/kg bw: 3/4 animals, 2 rabbits after 3 hours, 1 rabbit after 4 hours
40 mg/kg bw: 4/4 animals, 2 rabbits after 3 hours, 2 rabbits after 4 hours - Clinical signs:
- other: 6 mg/kg bw: 4/4 animals after 2 h: lethargy 25 mg/kg bw: 4/4 animals after 1.5 h lethary; 2/4 animals after 1,5 h: labored breathing; 1/4 animals after 3 h: convulsions 40 mg/kg bw: 4/4 animals after 1 h: lethargy; 3/4 animals (2 rabbits after 1 h, 1 rabb
- Gross pathology:
- No evidence of either external or internal lesions considered to be treatment-related.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 based on GHS criteria
- Remarks:
- Migrated information
- Conclusions:
- Legally binding harmonised classification as given in REGULATION (EC) No 1272/2008 Annex VI Table 3.1 (GHS): Acute tox. 1; H310 and Table 3.2 (Annex I of Directive 67/548/EEC): T+; R26/27/28.
- Executive summary:
In an acute dermal toxicity study equivalent to OECD TG 402 (1981) 3 groups of New Zealand White rabbits (2 males and 2 females per dose level) were dermally exposed to Acetone Cyanohydrin (98 wt % a.i., 10 % aq.) for 24 hours at doses of 6, 25, 40 mg/kg bw. Animals then were observed for 14 days.
Dermal LD50 Combined = 16 mg/kg bw (95% C.I. 8 - 28)
Lethargy (at 6, 25, 40 mg/kg bw), labored breathing (at 25 and 40 mg/kg bw), and convulsions (at 25 mg/kg bw) were clinical signs of toxicity observed after treatment. There were no treatment related necropsy findings. Slight (4/5 animals) to moderate (1/5 animals) redness, slight swelling in 3/5 animals, and slight necrosis in 1/5 animals were found after 24 hours post treatment in the surviving animals.
Legally binding harmonised classification as given in REGULATION (EC) No 1272/2008 Annex VI Table 3.1 (GHS):
Acute tox. 1; H310 and Table 3.2 (Annex I of Directive 67/548/EEC): T+; R26/27/28.
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