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EC number: 200-909-4 | CAS number: 75-86-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: - Basic data given: comparable to guidelines/standards
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Test Method: Ames B. (1975) Mutation Research 11, 347-364
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-hydroxy-2-methylpropionitrile
- EC Number:
- 200-909-4
- EC Name:
- 2-hydroxy-2-methylpropionitrile
- Cas Number:
- 75-86-5
- Molecular formula:
- C4H7NO
- IUPAC Name:
- 2-hydroxy-2-methylpropanenitrile
- Details on test material:
- - Name of test material (as cited in study report): 2-Hydroxy-2-methyl-propane-nitrile (Acetone cyanohydrine), form Aldrich Chemical
- Lable purity: 98+ %
- Code 160, coded by the National Toxicology Program (NTP) chemical repository (result table)
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium, TA100, TA1535, TA97, TA98
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-Mix fraction of Aroclor 1254-induced, male Sprague-Dawley rat and male Syrian hamster livers
- Test concentrations with justification for top dose:
- 0.3, 1.0, 10.0, 33.0, 100.0, 166.0 µg/plate
Each chemical was tested initially at half-log dose intervals up to a dose that elicited toxicity, or to a dose immediately below one which was toxic in the preliminary toxicity test. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: water
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Migrated to IUCLID6: for TA 1535, TA 100, without metabolic activation
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Migrated to IUCLID6: for TA 97 without metabolic activation
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylenediamine for TA 98 without metabolic activation
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene, with metabolic activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation assay as described by Haworth et al. 1983, with some differences.
- Salmonella typhimurium strains were obtained from Dr. Bruce Ames (University of California, Berkeley, U.S.A.) and were stored as recommended (Maron and Ames, 1983).
- Cultures were grown overnight with shaking at 37 °C in Oxoid No. 2 broth, and their phenotypes were analyszed prior to their use for mutagenicity assays.
- 300 chemicals were tested in several laboratories
Test protocol:
1) Testing in strains TA97, TA98, TA100, and TA1535 10% S-9 was used.
2) The first test of a chemical was without activation and with 10% S-9 in the S-9 mix. If a positive result was obtained the test was repeated. If the tests were negative they were repeated without S-9 and with 30% S-9.
3) The order of use of 10% and 30% S-9 was reversed.
4) Initial testing was in strains TA98 and TA100 without activation and with 30% rat and hamster S-9s. If a positive result was obtained in one of these
two strains it was repeated and the other strains were not used. If the tests were negative, the other strains were used with 30% and 10% S-9. A chemical was not designated nonmutagenic unless it had been tested in strains TA98, TA100, TA1535, and TA97 and/or TA1537, without activation and with 10% and 30% rat and hamster S-9. Ocasionally, 5% S-9 was also used in all protocol variations.
- Evaluation criteria:
- Evaluations were made at both the individual trial and overall chemical levels.
Individual trials were judged mutagenic (+), weakly mutagenic (+W), questionable (?), or nonmutagenic (-), depending on the magnitude of the increase of his + revertants, and the shape of the dose-response.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA 97, TA 98, TA 100, TA1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: other: Salmonella typhimurium, TA100, TA1535, TA97, TA98
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Up to 166 µg/plate, 2-Hydroxy-2 -methylpropanenitrile did not induce biologically relevant increases in revertants in any of the tested strains,in neither case with or without metablolic activation. - Executive summary:
In a reverse gene mutation assay in bacteria similar to OECD TG 471, four strains of S. typhimurium (TA 1535, TA 100, TA 98, and TA 97) were exposed to 2-Hydroxy-2 -methylpropanenitrile (solvent: water), at concentrations of 0.3, 1, 3, 10, 33, 100, 166 µg/plate in the presence and absence of mammalian metabolic activation (preincubation method).
2-Hydroxy-2 -methylpropanenitrile was tested up to cytotoxic concentrations.
The positive controls induced the appropriate responses in the corresponding strains. Up to 166 µg/plate, 2-Hydroxy-2 -methylpropanenitrile did not induce biologically relevant increases in revertants in any of the tested strains,in neither case with or without metablolic activation.
There was no evidence of induced mutant colonies over background.
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