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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2002-05-20 to 2002-07-24
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Concentrations: Samples of fresh and old test medium were taken at the start and end of the first (0-24 h) and and last (72-96h) exposure periods.

- Sampling method: Samples were taken from the centre of the test aquaria without mixing of the contents. The samples were deep frozen (-20ºC) immediately after collection.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: Test medium prepared by direct addition followed by stirring for 30 minutes.

- Differential loading: Single nominal exposure concentration of 100 mg/l

- Controls: Dilution water
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrafish

- Source: Zoohaus Schaub, Liestal, Switzerland

- Length at study initiation (length definition, mean, range and SD): 3.1 +/-0.1 cm

- Weight at study initiation (mean and range, SD): 0,26 +/-0.03 g

- Feeding during test: none

ACCLIMATION

- Acclimation period: >3 weeks

- Acclimation conditions (same as test or not): yes

- Type and amount of food: TetraMin®

- Feeding frequency: not reported

- Health during acclimation (any mortality observed): no fish died in the acclimation period and all appeared healthy
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
21 - 22ºC
Test temperature:
250 mg/l (as CaCO3)
pH:
7.9 - 8.0
Dissolved oxygen:
8.4 - 8.7 mg/l
Nominal and measured concentrations:
Nominal: Control and 100 mg/l

Measured concentrations in fresh and old 100 mg/l nominal treatment during the first (0-24 h) exposure period: 100 and 94.2 mg/l

Measured concentrations in fresh and old 100 mg/l nominal treatment during the fourth (72-96 h) exposure period: 117 and 92.5 mg/l

Mean measured concentration in 100 mg/l treatment: 101 mg/l

Mean measured concentration as a % of nominal: 101%
Details on test conditions:
TEST SYSTEM

- Test vessel: Aquarium

- Type: open

- Material, size, headspace, fill volume: Glass, 5 litre, 5 litre fill volume

- Aeration: slight

- Renewal rate of test solution (frequency/flow rate): daily

- No. of organisms per vessel: 7

- No. of vessels per concentration (replicates): 1

- No. of vessels per control (replicates): 1

- Biomass loading rate: 0.37 g/l wet weight


TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Reconstituted fresh water

- Ca/mg ratio: 4:1

- Culture medium different from test medium: no

- Intervals of water quality measurement: daily



OTHER TEST CONDITIONS

- Adjustment of pH: no

- Photoperiod: 16:8 (light:dark) with 30 min transition

- Light intensity: 50 - 500 Lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality observed after 2.5, 24, 48, 72 and 96 hours


TEST CONCENTRATIONS

- Spacing factor for test concentrations: not applicable

- Range finding study: yes

- Results used to determine the conditions for the definitive study: not reported
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: none

- Mortality of control: none

- Other adverse effects control: none

- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
Reported statistics and error estimates:
There wer no effects in the test and therefore statistical analysis of the results was not required.

There were no toxic effects observed in the test.

Validity criteria fulfilled:
yes
Conclusions:
A 96-h LC50 value of >100 mg/l and a NOEC of =100 mg/l have been determined for the effects of the test substance on mortality of Brachydanio rerio. It is likely that the test organisms were exposed primarily to the hydrolysis products of the substance.

Description of key information

Short-term toxicity to fish: 96 hr EC50 >100 mg/l (measured) (highest concentration tested) (EU C.1), in terms of the substance as tested. The observations in this study are attributed to the exposure of test organisms to the hydrolysis products in the test system.

Key value for chemical safety assessment

Additional information

A 96-h LC50 value of >100 mg/l and a NOEC of =100 mg/l (measured, highest concentration tested) have been determined for the effects of 3 -trimethoxysilylpropyl methacrylate (CAS 2530-85-0) based on mortality of Brachydanio rerio. It is likely that the test organisms were exposed primarily to the hydrolysis products of the substance.

The results of a second reliable test are also available. The test was conducted as a limit test at a concentration of 1042 mg/L and no effects on mortality were observed. The reliability of the results from a third test could not be determined because there was insufficient information in the source to do so.

 

Refer to the discussion in the Section 6 endpoint summary (Section 7.0 of CSR) for further discussion of the approach to chemical safety assessment for this registration substance.