3-trimethoxysilylpropyl methacrylate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002-05-20 to 2002-07-24

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Samples of fresh and old test medium were taken at the start and end of the first (0-24 h) and and last (72-96h) exposure periods.

- Sampling method: Samples were taken from the centre of the test aquaria without mixing of the contents. The samples were deep frozen (-20ºC) immediately after collection.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: Test medium prepared by direct addition followed by stirring for 30 minutes.

- Differential loading: Single nominal exposure concentration of 100 mg/l

- Controls: Dilution water

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish

- Source: Zoohaus Schaub, Liestal, Switzerland

- Length at study initiation (length definition, mean, range and SD): 3.1 +/-0.1 cm

- Weight at study initiation (mean and range, SD): 0,26 +/-0.03 g

- Feeding during test: none

ACCLIMATION

- Acclimation period: >3 weeks

- Acclimation conditions (same as test or not): yes

- Type and amount of food: TetraMin®

- Feeding frequency: not reported

- Health during acclimation (any mortality observed): no fish died in the acclimation period and all appeared healthy

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

21 - 22ºC

Test temperature:

250 mg/l (as CaCO3)

pH:

7.9 - 8.0

Dissolved oxygen:

8.4 - 8.7 mg/l

Nominal and measured concentrations:

Nominal: Control and 100 mg/l

Measured concentrations in fresh and old 100 mg/l nominal treatment during the first (0-24 h) exposure period: 100 and 94.2 mg/l

Measured concentrations in fresh and old 100 mg/l nominal treatment during the fourth (72-96 h) exposure period: 117 and 92.5 mg/l

Mean measured concentration in 100 mg/l treatment: 101 mg/l

Mean measured concentration as a % of nominal: 101%

Details on test conditions:

TEST SYSTEM

- Test vessel: Aquarium

- Type: open

- Material, size, headspace, fill volume: Glass, 5 litre, 5 litre fill volume

- Aeration: slight

- Renewal rate of test solution (frequency/flow rate): daily

- No. of organisms per vessel: 7

- No. of vessels per concentration (replicates): 1

- No. of vessels per control (replicates): 1

- Biomass loading rate: 0.37 g/l wet weight


TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Reconstituted fresh water

- Ca/mg ratio: 4:1

- Culture medium different from test medium: no

- Intervals of water quality measurement: daily



OTHER TEST CONDITIONS

- Adjustment of pH: no

- Photoperiod: 16:8 (light:dark) with 30 min transition

- Light intensity: 50 - 500 Lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality observed after 2.5, 24, 48, 72 and 96 hours


TEST CONCENTRATIONS

- Spacing factor for test concentrations: not applicable

- Range finding study: yes

- Results used to determine the conditions for the definitive study: not reported

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none

- Mortality of control: none

- Other adverse effects control: none

- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none

Reported statistics and error estimates:

There wer no effects in the test and therefore statistical analysis of the results was not required.

Sublethal observations / clinical signs:

There were no toxic effects observed in the test.

Validity criteria fulfilled:

yes

Conclusions:

A 96-h LC50 value of >100 mg/l and a NOEC of =100 mg/l have been determined for the effects of the test substance on mortality of Brachydanio rerio. It is likely that the test organisms were exposed primarily to the hydrolysis products of the substance.

Description of key information

96-hour LC₅₀ >100 mg/l (nominal, highest concentration tested) (EU C.1), Danio rerio. The LC₅₀ is equivalent to >83 mg/l when expressed in terms of the silanol hydrolysis product, 3-(trihydroxysilyl)propyl methacrylate.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 83 mg/L

Additional information

A 96-hour LC₅₀ value of >100 mg/l (nominal, highest concentration tested) has been determined for the effects of the registration substance, 3-trimethoxysilylpropyl methacrylate (CAS 2530-85-0, EC 219-785-8), on the mortality of Danio rerio (reported as: Brachydanio rerio) (Momentive, 2002a). Analysis of the tested substance by atomic absorption spectrophotometry confirmed that the mean measured concentrations did not deviate by more than 20% when compared to nominal concentrations. The test was conducted according to EU Method C.1 and in compliance with GLP. In view of the test media preparation method and exposure regime, it is likely that the test organisms were exposed predominantly to the hydrolysis products of the tested substance, 3-(trihydroxysilyl)propyl methacrylate and methanol.

The results may be expressed in terms of concentration of the silanol hydrolysis product, 3-(trihydroxysilyl)propyl methacrylate, by applying a molecular weight correction: (MW of silanol = 206.27 / MW of parent = 248.35) * >100 = >83 mg/l.

This value is supported by two short-term fish toxicity studies. A reliable limit test was conducted at a concentration of 1042 mg/l to Danio rerio for 96-hours' exposure, and no effects on mortality were observed (Evonik, 2000a). Another study reported an LC₅₀ value of 252 mg/l for Pimephales promelas, however the reliability of the results could not be determined because there was insufficient information in the source (Momentive, 1997a).