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Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995-09-20 to 1995-10-04
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on preparation and application of test substrate:
- Controls: Untreated soil
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM

- Common name: Earthworm, Eisenia foetida foetida

- Source: Eisenia GmbH und Co, Wiesbaden, Germany

- Age at test initiation (mean and range, SD): 2 months

- Weight at test initiation (mean and range, SD): 439 mg (303-565) in Control and 452 mg (313-581 in treatment).
Study type:
laboratory study
Substrate type:
other: quartz sand
Limit test:
yes
Total exposure duration:
14 d
Test temperature:
20 +/-2 ºC
pH:
6.6-6.8
Moisture:
40% of holding capacity
Details on test conditions:
TEST SYSTEM

- No. of organisms per container (treatment): 10

- No. of replicates per treatment group: 4

- No. of replicates per control: 4


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)

- Composition (if artificial substrate): Quartz sand


OTHER TEST CONDITIONS

- Photoperiod: Daylight

- Light intensity: ca. 400-600 Lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality after 7 and 14 days exposure


VEHICLE CONTROL PERFORMED: no


TEST CONCENTRATIONS

- Limit test at 1000 mg/kg
Nominal and measured concentrations:
Nominal: 1000 mg/kg
Reference substance (positive control):
yes
Remarks:
Chloracetamide
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
other: hydrolysis products
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
other: hydrolysis products
Basis for effect:
mortality
Details on results:
- Mortality at end of exposure period: 0
Results with reference substance (positive control):
After 7 and 14 days exposure mortality was 0 in the 30 mg/kg treatment and 100% in the 100 mg/kg treatment.
Reported statistics and error estimates:
There were no toxic effects observed in the test and therefore statistical analysis of the test results was not required.

No toxic effects were observed in the test.

Validity criteria fulfilled:
yes
Conclusions:
A 14-day LC50 value of >1000 mg/kg dry weight and NOEC of =1000 mg/kg dry weight have been determined for the effects of the test substance on mortality of Eisenia foetida. It is likely that the test organisms were exposed primarily to the hydrolysis products of the test substance.
Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Data waiving:
study scientifically not necessary / other information available
Justification for data waiving:
other:
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information
Reason / purpose for cross-reference:
data waiving: supporting information

Description of key information

14-day LC50 >1000 mg/kg dry weight (nominal, highest concentration tested) (EU A.8), Eisenia fetida. The LC50 is equivalent to >830 mg/l when expressed in terms of the silanol hydrolysis product, 3-(trihydroxysilyl)propyl methacrylate.

Key value for chemical safety assessment

Additional information

A 14-day LC50 value of >1000 mg/kg dry weight and a NOEC value of =1000 mg/kg dry weight (nominal, highest concentration tested) have been determined for the effects of the registration substance, 3-trimethoxysilylpropyl methacrylate (CAS 2530-85-0, EC 219-785-8), on the mortality of Eisenia fetida (Evonik, 1995). The test was conducted according to EU Method A.8 and in compliance with GLP. In view of the test media preparation method and exposure regime, it is likely that the test organisms were exposed predominantly to the hydrolysis products of the tested substance, 3-(trihydroxysilyl)propyl methacrylate and methanol.

The results may be expressed in terms of concentration of the silanol hydrolysis product, 3-(trihydroxysilyl)propyl methacrylate, by applying a molecular weight correction: (MW of silanol = 206.27 / MW of parent = 248.35) * >1000 = >830 mg/kg dry weight.