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EC number: 200-835-2 | CAS number: 75-05-8
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 23 October 1995 - 29 March 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test and Reproduction Test) (1984)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Physical state: colourless and transparent liquid
- Analytical purity: 99.99% (GC)
- Lot/batch No.: 5L5051
- Stability under test conditions: Stable
- Storage condition of test material: In the refrigerator - Analytical monitoring:
- yes
- Details on sampling:
- - Sampling:
New: freshly prepared test solution
Old: test solutions 1, 2 or 3 days after freshly prepared
- Sampling volume: 5.0 mL
New: 0, 5, 10, 17 day
Old: 3, 7, 11, 18 day - Vehicle:
- not specified
- Details on test solutions:
- A set of test solutions used in this study were prepared by diluting a stock solution (40000 mg/L), with the dechlorinated tap water.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: National Institute for Environmental Studies, Japan
- Feeding during test
- Food type: Chlorella vulgaris
- Amount: 0.1 - 0.2mgC/daphnia/day
ACCLIMATION
- Acclimation period: 3 or 4 weeks
- Acclimation conditions: same as test
- Type and amount of food: Chlorella vulgaris - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Test temperature:
- 20.0 - 20.8℃
- pH:
- 7.6 - 8.1
- Dissolved oxygen:
- 7.0 - 10.4 mg/L
- Nominal and measured concentrations:
- nominal: 0, 100, 320, 1000 mg/L
measured (time weighted average): 0, 92, 300, 960 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: deep petri dish
- Type: closed
- Material: glass
- size: 850 mL
- headspace: No
- fill volume: 850 mL
- Renewal rate of test solution: 0, 3, 5, 7, 9 day, everyday between 9 and 21 day
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Hardness of dilution water: 50 mg/L (as CaCO3)
- pH of dilution water: 7.9
OTHER TEST CONDITIONS
- Photoperiod: 16h light/8h dark
- Light intensity: At room light
RANGE-FINDING STUDY
- Test concentrations: acute toxicity study (48h); 1000 mg/L
- Results used to determine the conditions for the definitive study: 48h-EiC50 > 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 960 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 960 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 960 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of parent animals: 10% (control), 2.5% (92 mg/L), 5% (300 mg/L), 37.5% (960 mg/L) at the end of the test
- Total number of living offspring produced per parent animal (average): 41 (control), 43 (92 mg/L), 35 (300 mg/L), 32 (960 mg/L)
- Body length and weight of parent animals: No difference
- First delivery: 8 day (control, 92, mg/L, 300 mg/L), 8 or 9 day (960 mg/L) - Results with reference substance (positive control):
- - 48h EC50: 0.14 mg/L
- Reported statistics and error estimates:
- LC50, EC50: Not applicable
NOEC, LOEC: Dunnett's multiple comparison test - Conclusions:
- The 21-day NOEC (reproduction) for acetonitrile in Daphna magna is concluded to be 960 mg/L (measured) under semi-static exposure conditions.
- Executive summary:
In a guideline (OECD 202) and GLP study, MCSI of Japan (1996) reported the 21 -day NOEC (reproduction) for acetonitrile in Daphna magna to be 960 mg/L (measured) under semi-static exposure conditions.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guidelines for Testing Chemicals (OECD 1987)
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Aquatic Organism Institute, Chinese National Academy of Science.
- Age of parental stock: less than 24 hours
- Feeding during test: Selenastrum capricornutam at the concentration of 5.0-6.0 x 10 cell/mL - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Remarks on exposure duration:
- single generation
- Hardness:
- Dilution water - 1.86 +/- 0.08 mg (CaCO3)/L
- Test temperature:
- 24 +/- 1 degree C
- pH:
- Dilution water - 6.5-7.5
- Dissolved oxygen:
- Dilution water - 7.5-8.0 mg/L
Test Solution - > 5 mg/L - Nominal and measured concentrations:
- 80, 160, 320, 640 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 mL beaker
- Material, size, headspace, fill volume: 100 test solution
- No. of organisms per vessel: 3
- No. of vessels per concentration: 4
- No. of vessels per control: 4
- Renewal rate of test solution (frequency/flow rate): renewed once per day
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water dechlorinated by active carbon
- Intervals of water quality measurement: beginning and the end of each test by the standard methods (APHA-AWWA-WPCF 1985).
OTHER TEST CONDITIONS
- Adjustment of pH: the deviation of pH was less 0.3
- Photoperiod: 16 hr light/8 hr dark
- Light intensity: density was 2000 Lux - Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 160 mg/L
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 320 mg/L
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- other: ChV
- Effect conc.:
- 230 mg/L
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- other: ACR
- Effect conc.:
- 16 other: ACR
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 640 mg/L
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1 300 mg/L
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- other: ChV
- Effect conc.:
- > 900 mg/L
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- other: ACR
- Effect conc.:
- < 4 other: ACR
- Basis for effect:
- mortality
- Details on results:
- - Mortality of control: 8%
- Reproductive induces: mean number per female in test group was 48.8 and in the control group 46.2 - Conclusions:
- The results indicted that reproduction was more sensitive than survival in both 14-d and 21-d tests for two pollutants.
- Executive summary:
Tong et al (1996) reported a 21 -day, static renewal NOEC of 160 mg/L for acetonitrile in Water flea, (Daphnia magna), based on reproductive performance measures.
Referenceopen allclose all
Survival rate in the control group was 92% at the end of test.
Survival and reproduction of D. magna during l4-d and 21-d chronic tests
| Chemical | Concentration mg/L | Survival (%) | Reproduction * | ||
| 21 - d | 14 - d | 21 - d | 14 - d | ||
| Acetonitrile | Control | 92 | 92 | 244 | 148 |
| 80 | 92 | 92 | 234 | 151 | |
| 160 | 100 | 100 | 218 | 140 | |
| 320 | 92 | 100 | 208 | 129 | |
| 640 | 67 | 83 | 199 | 119 | |
* The number of neonates per female.
Description of key information
21-day NOEC (reproduction) values of 160 mg/L and >960 mg/L have been reported for acetonitrile in Daphnia magna.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 160 mg/L
Additional information
In a guideline (OECD 202) and GLP study, MCSI of Japan (1996) reported the 21 -day NOEC (reproduction) for acetonitrile in Daphna magna to be >960 mg/L (measured) under semi-static exposure conditions. Tong et al (1996) also studied the chronic toxicity of acetonitrile in Daphnia magna. These investigators reported a 21 -day, static renewal NOEC of 160 mg/L for acetonitrile in Daphnia magna, based on reproductive performance measures.
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