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EC number: 200-835-2 | CAS number: 75-05-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Principles of method if other than guideline:
- This study was conducted in accordance with Standard Operating Procedures, Good Laboratory Practice Standards as set forth by the EPA in 40 CFR Part 160 (FIFRA) and 40 CFR Part 792 (TSCA), JMAFF in NohSan No. 3850, and OECD in (C(81)30(Final)Annex 2), and the Protocol as approved by the Sponsor. Procedures pertinent to this study are described in this report.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Acetonitrile
- EC Number:
- 200-835-2
- EC Name:
- Acetonitrile
- Cas Number:
- 75-05-8
- Molecular formula:
- C2H3N
- IUPAC Name:
- acetonitrile
- Details on test material:
- - Name of test material (as cited in study report): Acetonitrile HPLC Grade
- Physical state: Clear liquid
- Analytical purity: 99.9%
- Impurities (identity and concentrations): Water: 0.002%
- Lot/batch No.: 973432
- Storage condition of test material: Room temperature
- Supplier: Fisher Chemicals, Fair Lawn, New Jersey
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Acetonitrile HPLC Grade
- Physical state: Clear liquid
- Analytical purity: 99.9%
- Impurities (identity and concentrations): Water: 0.002%
- Lot/batch No.: 973432
- Storage condition of test material: Room temperature
- Supplier: Fisher Chemicals, Fair Lawn, New Jersey
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Covance Research Products, Inc., Kalamazoo, Michigan.
- Age at study initiation: 7.5 months of age
- Weight at study initiation: The body weight range on the day of dosing was 3283-3519 grams.
- Housing: housed in individual stainless steel slatted floor cages in an animal room
- Diet: Certified Rabbit Chow® # 5322, PMI Feeds, Inc., St. Louis, Missouri was available up to 125 g/day.
- Water: ad libitum
- Acclimation period: The animals were maintained in accordance with the recommendations contained in the D.H.H.S. Publication entitled "Guide for the Care and Use of Laboratory Animals" and conditioned for a period of 114 days prior to dosing.
ENVIRONMENTAL CONDITIONS
- Temperature: 66-68°F
- Humidity: 56-66%
- Photoperiod: 12 hours light and 12 hours dark
IN-LIFE DATES: From: May 12,1997 To: September 8, 1997
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- shaved
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 mL - Duration of treatment / exposure:
- 4 hours
- Observation period:
- 72 hours after bandage removal
- Number of animals:
- 6 male
- Details on study design:
- Each rabbit was identified by a Monel® metal ear tag bearing the individual animal number.
TEST SITE
- Area of exposure: 1 intact skin site on the back of each rabbit. Dorsal area
- Type of wrap if used: The test sites were then wrapped with 8-ply gauze bandaging and overwrapped with Denniform® tape. A collar (E-Jay Saf-T-Shield) was placed on each rabbit to prevent them from disturbing the application site and wrapping.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Following the exposure period, the bandaging materials and collars were removed and the test sites were wiped with disposable toweling moistened with water to remove any residual material.
SCORING SYSTEM: Draize
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Irritant / corrosive response data:
- The test sites were evaluated for dermal irritation approximately 0.5-1, 24, 48, and 72 hours following patch removal and scored based on the Draize method. All scores at each observation interval were 0 for each animal.
- Other effects:
- No signs of ill health or test article-related effects were observed during the study.
Any other information on results incl. tables
The overall irritation score was 0.0
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Based on the results of this study, Acetonitrile (HPLC Grade) is non-irritating to the skin of rabbits.
- Executive summary:
In a guideline (OECD 404 equivalent) and GLP study, Acetonitrile (HPLC Grade) was found to be non-irritating to the skin of rabbits (4 -hour exposure). All scores at each observation interval were 0.0 for each animal. No other signs of ill health or test article-related effects were observed during the study.
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