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EC number: 403-530-4 | CAS number: 129423-54-7 PV-ECHTGELB HGR
- Life Cycle description
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- Endpoint summary
- Appearance / physical state / colour
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- Density
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- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study according to OECD Guideline No. 201 and GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: samples were diluted 1:20 with mobile phase and analysed.
- Sample storage conditions before analysis: all samples were stored at 7 ± 2°C until start of analysis - Vehicle:
- no
- Details on test solutions:
- PREPARATION OF TEST SOLUTION
A saturated solution was used as limit concentration based on the results of a range finding test. A dispersion of 53 mg/L, prepared in dilution water, was intensively stirred(20500 rpm) for 5 min and membrane filtrated with 0.45µm (RC55, S&S 10410214).
Limit concentration measured: 39.8 mg/L (mean) - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: Desmodesmus subspicatus CHODAT SAG 86.81
- Source (laboratory, culture collection): Sammlung von Algenkulturen (SAG), Pflanzenphysiologisches Institut der Universität Göttingen, Göttingen, Germany
- Age of inoculum (at test initiation): four day-old preculture
- Method of cultivation: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted 35-70 μE·m-2·s-1 for 24 h per day.
ACCLIMATION
- Acclimation period: four days, see above
- Culturing media and conditions (same as test or not): preculture incubated in 500 mL Erlenmeyer flasks; all algae were from the same source.
- Any deformed or abnormal cells observed: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 22-23 (mean: 22.5) °C
- pH:
- Start: 8.09 (test solution), 8.33 (control)
End, 72 h: 9.01 (test solution), 9.14 (control) - Nominal and measured concentrations:
- A limit test with a saturated solution 39.8 mg/L (mean) was carried out.
Measured concentrations: test start 39.4 mg/L, test end 40.1 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Sterile erlenmeyer flasks, volume: 250 mL
- Test volume: 100 mL
- Initial cells density: appr. 10000 cells/mL
- Control end cells density: 1196245 (mean from 6 replicates) cells/mL
- No. of vessels per limit concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water according to guidelines
- Intervals of water quality measurement: at the start and at the end (72 h)
OTHER TEST CONDITIONS
- Sterile test conditions: yes (sterile erlenmeyer flasks were used)
- Photoperiod: 24 h/d light
- Light intensity and quality: min. 60.8, max. 72.4 (mean 68.1 μE·m-2·s-1)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting in a plankton chamber
- Other: measurements at the beginning of the test (after application) and every 24 h, filtrated culture medium was used as ground signal. Each replicate was measured 6-fold.
TEST CONCENTRATIONS
- Test concentrations: 39.8 mg/L
- Spacing factor for test concentrations: only one concentration (saturated solution), limit test
- Justification for using less concentrations than requested by guideline: limit test - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate p.a.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 39.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: not applicable
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 39.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: not applicable
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 39.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: biomass and growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 39.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: biomass and growth rate
- Details on results:
- NOEC, LOEC and EC50 values for Inhibition of biomass growth and rate-related inhibition.
- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
Environmental conditions, pH-value, measured at 0 and 72 h, and room temperature, measured continuously, met the validity criteria of the guideline. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50 (biomass growth): 0.29 mg/L (0-72 h), 95 % confidence interval: 0.26-0.33 mg/l
- EC50 (rate-related inhibition): 0.59 mg/L (0-72 h), 95 % confidence interval: 0.55-0.63 mg/l - Reported statistics and error estimates:
- The NOEC and LOEC were determined by calculation of statistical significance of biomass integrals and growth rates. One Way Analysis of Variance (ANOVA) and DUNNETT’s test was carried out for the determination of statistically significant differences compared to control replicates. When running a One Way Analysis of Variance a Normality Test and an Equal Variance Test were done first. P-values for both Normality and Equal Variance Tests were 0.05. The α-value for ANOVA was α=0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No inhibiting effects on biomass growth and specific growth rate were found in the saturated solution. The EC50-values for inhibition of biomass growth (EbC50) and specific growth rate (ErC50) after 72 h were > 39.8 mg/L (mean).
- Executive summary:
The toxicity of Pigment Yellow 191 to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD-Guideline 201. The aim of the study was to assess the effects on growth rate and biomass production over a period of 72 h. The study was conducted under static conditions with an initial cell density of appr. 10000 cells/mL. A limit test with a saturated solution was carried out. A solution of 39.8 mg/L test item was prepared. The limit concentration was measured at the beginning and the end of the study. Three replicates were tested for the saturated solution and 6 replicates for the control. Environmental conditions, pH-value and room temperature, were determined to be within the acceptable limits. All effect values are based on the saturated solution of the test item.
In this study the test item did not cause any inhibiting effects to Desmodesmus subspicatus after 72 h when tested with a 39.8 mg/L solution.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reason / purpose for cross-reference:
- read-across source
- GLP compliance:
- yes
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 39.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: not applicable
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 39.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: not applicable
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 39.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: biomass and growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 39.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: biomass and growth rate
- Details on results:
- NOEC, LOEC and EC50 values for Inhibition of biomass growth and rate-related inhibition.
- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
Environmental conditions, pH-value, measured at 0 and 72 h, and room temperature, measured continuously, met the validity criteria of the guideline. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50 (biomass growth): 0.29 mg/L (0-72 h), 95 % confidence interval: 0.26-0.33 mg/l
- EC50 (rate-related inhibition): 0.59 mg/L (0-72 h), 95 % confidence interval: 0.55-0.63 mg/l - Reported statistics and error estimates:
- The NOEC and LOEC were determined by calculation of statistical significance of biomass integrals and growth rates. One Way Analysis of Variance (ANOVA) and DUNNETT’s test was carried out for the determination of statistically significant differences compared to control replicates. When running a One Way Analysis of Variance a Normality Test and an Equal Variance Test were done first. P-values for both Normality and Equal Variance Tests were 0.05. The α-value for ANOVA was α=0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No inhibiting effects on biomass growth and specific growth rate were found in the saturated solution. The EC50-values for inhibition of biomass growth (EbC50) and specific growth rate (ErC50) after 72 h were > 39.8 mg/L (mean).
- Executive summary:
The toxicity of Pigment Yellow 191 to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD-Guideline 201. The aim of the study was to assess the effects on growth rate and biomass production over a period of 72 h. The study was conducted under static conditions with an initial cell density of appr. 10000 cells/mL. A limit test with a saturated solution was carried out. A solution of 39.8 mg/L test item was prepared. The limit concentration was measured at the beginning and the end of the study. Three replicates were tested for the saturated solution and 6 replicates for the control. Environmental conditions, pH-value and room temperature, were determined to be within the acceptable limits. All effect values are based on the saturated solution of the test item.
In this study the test item did not cause any inhibiting effects to Desmodesmus subspicatus after 72 h when tested with a 39.8 mg/L solution.
Referenceopen allclose all
Description of key information
The toxicity of Pigment Yellow 191 to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD-Guideline 201. The aim of the study was to assess the effects on growth rate and biomass production over a period of 72 h. The study was conducted under static conditions with an initial cell density of approximately 10000 cells/mL. A limit test with a saturated solution was carried out. A solution of 39.8 mg/L test item was prepared. The limit concentration was measured at the beginning and the end of the study. Three replicates were tested for the saturated solution and 6 replicates for the control. Environmental conditions, pH-value and room temperature, were determined to be within the acceptable limits. All effect values are based on the saturated solution of the test item.No inhibiting effects on biomass growth and specific growth rate were found in the saturated solution. The EC50-values for inhibition of biomass growth (EbC50) and specific growth rate (ErC50) after 72 h were > 39.8 mg/L (mean).
he toxicity of Pigment Yellow 191 to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD-Guideline 201. The aim of the study was to assess the effects on growth rate and biomass production over a period of 72 h. The study was conducted under static conditions with an initial cell density of approximately 10000 cells/mL. A limit test with a saturated solution was carried out. A solution of 39.8 mg/L test item was prepared. The limit concentration was measured at the beginning and the end of the study. Three replicates were tested for the saturated solution and 6 replicates for the control. Environmental conditions, pH-value and room temperature, were determined to be within the acceptable limits. All effect values are based on the saturated solution of the test item.
No inhibiting effects on biomass growth and specific growth rate were found in the saturated solution. The EC50-values for inhibition of biomass growth (EbC50) and specific growth rate (ErC50) after 72 h were > 39.8 mg/L (mean).
he toxicity of Pigment Yellow 191 to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD-Guideline 201. The aim of the study was to assess the effects on growth rate and biomass production over a period of 72 h. The study was conducted under static conditions with an initial cell density of approximately 10000 cells/mL. A limit test with a saturated solution was carried out. A solution of 39.8 mg/L test item was prepared. The limit concentration was measured at the beginning and the end of the study. Three replicates were tested for the saturated solution and 6 replicates for the control. Environmental conditions, pH-value and room temperature, were determined to be within the acceptable limits. All effect values are based on the saturated solution of the test item.
No inhibiting effects on biomass growth and specific growth rate were found in the saturated solution. The EC50-values for inhibition of biomass growth (EbC50) and specific growth rate (ErC50) after 72 h were > 39.8 mg/L (mean).
he toxicity of Pigment Yellow 191 to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD-Guideline 201. The aim of the study was to assess the effects on growth rate and biomass production over a period of 72 h. The study was conducted under static conditions with an initial cell density of approximately 10000 cells/mL. A limit test with a saturated solution was carried out. A solution of 39.8 mg/L test item was prepared. The limit concentration was measured at the beginning and the end of the study. Three replicates were tested for the saturated solution and 6 replicates for the control. Environmental conditions, pH-value and room temperature, were determined to be within the acceptable limits. All effect values are based on the saturated solution of the test item.
No inhibiting effects on biomass growth and specific growth rate were found in the saturated solution. The EC50-values for inhibition of biomass growth (EbC50) and specific growth rate (ErC50) after 72 h were > 39.8 mg/L (mean).
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 39.8 mg/L
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