reaction products of diglycidyl ether bisphenol F (DGEBF) and oligomeric phenol diglycidyl ethers with acrylic acid

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented publication which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

The mutagenicity of mono- and di-functional aromatic glycidyl compounds

Author:

Seiler, JP

Year:

1984

Bibliographic source:

Mutation Research, 135 (1984) 159-167

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

ICR mice were treated orally with a single dose of the test substance. After 24, 48, 72 h bone marrow cells were prepared and evaluated for of micronucleated polychromatic erythrocytes.

GLP compliance:

not specified

Type of assay:

micronucleus assay

Test material

Test material information

Constituent 1

Reference substance name:

Resorcinol diglycidyl ether

IUPAC Name:

Resorcinol diglycidyl ether

Constituent 2

Reference substance name:

m-bis(2,3-epoxypropoxy)benzene

EC Number:

202-987-5

EC Name:

m-bis(2,3-epoxypropoxy)benzene

Cas Number:

101-90-6

IUPAC Name:

2,2'-[1,3-phenylenebis(oxymethylene)]dioxirane

Details on test material:

- Name of test material (as cited in study report): Resorcinol diglycidyl ether (RDGE)
- Analytical purity: >98%

Test animals

Species:

mouse

Strain:

ICR

Sex:

male/female

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

- Vehicle(s)/solvent(s) used: polyethylene glycol (PEG 400)

Duration of treatment / exposure:

24, 48, 72 h

Frequency of treatment:

single treatment

Doses / concentrations

Remarks:

Doses / Concentrations:
300, 600 mg/kg bw
Basis:
actual ingested

Examinations

Tissues and cell types examined:

bone marrow erythrocytes

Results and discussion

Test results

Sex:

male/female

Genotoxicity:

negative

Toxicity:

yes

Remarks:

tested up to acutely toxic levels

Any other information on results incl. tables

 

	frequency (± SD) of micronucleated erythrocytes per 1000 polychromatic erythrocytes at dose level:
fixation time [h]	300 mg/kg bw	600 mg/kg bw
24	0.0 (1.5)	0.0 (1.9)
48	not tested	0.9 (1.3)
72	not tested	0.4 (0.6)

 

  

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): negative
In this mouse bone marrow micronucleus assay, Resorcinol diglycidyl ether did not induce an increase in the frequency of micronucleated polychromatic erythrocytes.

Executive summary:

In an ICR mouse bone marrow micronucleus assay, male and female mice were treated orally with Resorcinol diglycidyl ether at doses of 300 and 600 mg/kg bw.  Bone marrow cells were harvested at 24 h (300 and 600 mg/kg bw), 48 and 72 h (600 mg/kg bw only) post-treatment.  The vehicle was polyethylene glycol (PEG 400). Animals were treated up to acutely toxic levels (however, signs of toxicity are not described in the publication).

There was no significant increase in the frequency of micronucleated polychromatic erythrocytes in bone marrow after any treatment time and dose level.