Registration Dossier
Registration Dossier
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EC number: 204-289-6 | CAS number: 118-96-7
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian cell study: DNA damage and/or repair
- Remarks:
- Type of genotoxicity: DNA damage and/or repair
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 985
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 486 (Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.39 (Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells In Vivo)
- GLP compliance:
- yes
- Type of assay:
- unscheduled DNA synthesis
Test material
- Reference substance name:
- 2,4,6-trinitrotoluene
- EC Number:
- 204-289-6
- EC Name:
- 2,4,6-trinitrotoluene
- Cas Number:
- 118-96-7
- Molecular formula:
- C7H5N3O6
- IUPAC Name:
- 2-methyl-1,3,5-trinitrobenzene
- Details on test material:
- TNT, > 99% purity
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Alderley Park (AP) or Fischer 344 (F344)
- Sex:
- male
Administration / exposure
- Route of administration:
- oral: gavage
- Duration of treatment / exposure:
- 12 hours
- Frequency of treatment:
- adminitered once
Doses / concentrationsopen allclose all
- Dose / conc.:
- 100 mg/kg bw/day (actual dose received)
- Dose / conc.:
- 200 mg/kg bw/day (actual dose received)
- Dose / conc.:
- 500 mg/kg bw/day (actual dose received)
- Dose / conc.:
- 1 000 mg/kg bw/day (actual dose received)
- No. of animals per sex per dose:
- 2, 3 or 5 animals per dose
- Control animals:
- yes
Examinations
- Tissues and cell types examined:
- Liver, blood.
Results and discussion
Test results
- Key result
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- no effects
Any other information on results incl. tables
TNT was inactive in each strain. Urine of the TNT-treated animals esd bright red due to the presence of TNT (in an acid medium) and their blood contained elevated levels of methaemoglobin. Nonetheless, hepatocyte, morphology was normal and showed no evidence of toxic picnosis.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative.
Negative response observed for TNT in the liver assay indicates that it is unlikely to be rat hepatocarcinogen. Nonetheless, high levels, of methaemoglobin were observed in the TNT-trerated rats and their urine was coloured red. These facts, together with the known toxicities of this agent suggest a possible carcinogenic hazard to the haemopoetic and urinary tissue of animals exposed chronically to it at toxic dose-levels.
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