Registration Dossier
Registration Dossier
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EC number: 235-008-5 | CAS number: 12054-48-7
Ecotoxicological information
Toxicity to microorganisms
Currently viewing:
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given, acceptable puplication for assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Detection of heavy metal toxicity and genotoxicity in wastewaters by microbial assay
- Author:
- Codina et al.
- Year:
- 1 994
- Bibliographic source:
- Water Sci. Tech., Vol. 30, pp: 145-151
Materials and methods
- Principles of method if other than guideline:
- Five differend acute toxicity standard tests were used
- GLP compliance:
- no
Test material
- Reference substance name:
- Nickel dichloride
- EC Number:
- 231-743-0
- EC Name:
- Nickel dichloride
- Cas Number:
- 7718-54-9
- IUPAC Name:
- nickel(2+) dichloride
- Reference substance name:
- Nickel chloride
- EC Number:
- 253-399-0
- EC Name:
- Nickel chloride
- Cas Number:
- 37211-05-5
- IUPAC Name:
- nickel(2+) dichloride
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution of NiCl2 was optained by dissolving them in deionized water or sterilized wastewater and stored at 4 °C
Test organisms
- Test organisms (species):
- other: Pseudomonas fluorescens and bakers yeast
- Details on inoculum:
Pseudomonas fluorescens CECT-385
- Laboratory culture: yes
Bakers yeast
- Laboratory culture: yes
Study design
- Test type:
- static
- Water media type:
- freshwater
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 1 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6 151 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: DEIONIZED WATER: BYS: respiration inhibition test with bakers yeast (spectrophotometric method)
- Duration:
- 1 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9 536.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: WASTE WATER: BYS: respiration inhibition test with bakers yeast (spectrophotometric method)
- Duration:
- 1 h
- Dose descriptor:
- EC50
- Effect conc.:
- 763 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: DEIONIZED WATER: PFS: respiration inhibition test with P. fluorescens (spectrophotometric method)
- Duration:
- 1 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 301.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: WASTE WATER: PFS: respiration inhibition test with P. fluorescens (spectrophotometric method)
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 53.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: DEIONIZED WATER: PFP: growth inhibition test with P. fluorescens (plate)
- Duration:
- 15 min
- Dose descriptor:
- EC50
- Effect conc.:
- 55.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: luminous activity
- Remarks on result:
- other: DEIONIZED WATER: Microtox-test
- Duration:
- 15 min
- Dose descriptor:
- EC50
- Effect conc.:
- 170.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: luminous activity
- Remarks on result:
- other: WASTE WATER: Microtox test
- Duration:
- 1 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2 399 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Nickeldihydroxid
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: DEIONIZED WATER: BYS: respiration inhibition test with bakers yeast (spectrophotometric method). Result calculated for Nickeldihydroxid
- Duration:
- 1 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3 719.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Nickeldihydroxid
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: WASTE WATER: BYS: respiration inhibition test with bakers yeast (spectrophotometric method). Result calculated for Nickeldihydroxid
- Duration:
- 1 h
- Dose descriptor:
- EC50
- Effect conc.:
- 297.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Nickeldihydroxid
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: DEIONIZED WATER: PFS: respiration inhibition test with P. fluorescens (spectrophotometric method). Result calculated for Nickeldihydroxid
- Duration:
- 1 h
- Dose descriptor:
- EC50
- Effect conc.:
- 507.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Nickeldihydroxid
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: WASTE WATER: PFS: respiration inhibition test with P. fluorescens (spectrophotometric method). Result calculated for Nickeldihydroxid
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 20.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Nickeldihydroxid
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: DEIONIZED WATER: PFP: growth inhibition test with P. fluorescens (plate). Result calculated for Nickeldihydroxid
- Duration:
- 15 min
- Dose descriptor:
- EC50
- Effect conc.:
- 21.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Nickeldihydroxid
- Basis for effect:
- other: luminous activity
- Remarks on result:
- other: DEIONIZED WATER: Microtox-test. Result calculated for Nickeldihydroxid
- Duration:
- 15 min
- Dose descriptor:
- EC50
- Effect conc.:
- 66.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Nickeldihydroxid
- Basis for effect:
- other: luminous activity
- Remarks on result:
- other: WASTE WATER: Microtox-test. Result calculated for Nickeldihydroxid
Any other information on results incl. tables
Results from the Netherland standard test (NEN6509) not available.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
