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EC number: 215-925-7 | CAS number: 1453-58-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Auto flammability
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- Endpoint summary
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
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- Toxicological Summary
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- Additional toxicological data

Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-06-06 - 2012-11-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- 3-methylpyrazole
- EC Number:
- 215-925-7
- EC Name:
- 3-methylpyrazole
- Cas Number:
- 1453-58-3
- Molecular formula:
- C4H6N2
- IUPAC Name:
- 3-methyl-1H-pyrazole
- Test material form:
- other: liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- CD-1
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- Species / Strain / Stock Rat (Rattus norvegicus) / CD / Crl: CD(SD)
Supplier Charles River Laboratories,
Research Models and Services,
Germany GmbH
Sandhofer Weg 7
97633 Sulzfeld
Germany
Selection of species In accordance with OECD 423; the rat is the preferred species
Sex Female
Number of animals 9 female animals
Groups 6 animals for the dose levels of 300 mg/kg b.w., 3 animals for the dose level of 2000 mg/kg b.w.
Body weight
(at start of administration) 176 - 209 g
Age
(at start of administration) Approx. 8 weeks
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 0.8% aqueous hydroxypropylmethylcellulose (for the dose level of 300 mg/kg only)
- Doses:
- 300 and 2000 mg/kg b.w.
- No. of animals per sex per dose:
- 6 animals for the dose levels of 300 mg/kg b.w., 3 animals for the dose level of 2000 mg/kg b.w.
- Control animals:
- no
- Details on study design:
- Principle of the ATC-test method
This procedure permits the identification of the 'acute-toxic-class' (ATC), a measurement of the acute toxicity by the oral route.
The test item is administered orally by gavage at a single dose level to a group of experimental animals. The dose used is selected from a series of defined dose levels. Due to the small number of animals used with this method, there is no need to perform a range finding test.
The test item is tested using a stepwise procedure, each step uses three female animals. The results of each step determine if:
o no further testing is needed,
o the next step will be performed with the same dose,
o the next step will be performed at the next higher or next lower dose level.
Starting at 2000 mg/kg b.w.
o Testing at 2000 mg/kg b.w.:
Three animals of one sex (preferably females) are treated at 2000 mg/kg b.w. (first step). If two to three animals die, testing at 300 mg/kg b.w. should be performed.
If no to one animal dies, the test item should be retested (second step) with 2000 mg/kg b.w., using three animals of the same sex.
If, in this second step, two to three animals die, testing at 300 mg/kg b.w. should be performed. If, in this second step, no to one animal dies, no further testing is necessary.
o Testing at 300 mg/kg b.w.:
If the results of the test at 2000 mg/kg b.w. indicate the need for further testing at a lower dose level.
Three female animals are treated at 300 mg/kg b.w. (first step).
If two or three animals die, testing at 50 mg/kg b.w. should be performed.
If fewer than two animals die, the test item should be retested (second step) with 300 mg/kg b.w., using three animals of the same sex.
If, in this second step, two or three animals die, testing at 50 mg/kg b.w. should be performed. If, in this second step, no to one animal dies, no further testing is necessary.
o Testing at 50 mg/kg b.w.:
If the results of the test at 300 mg/kg b.w. indicate the need for further testing at a lower dose level.
Three female animals treated at 50 mg/kg b.w. (first step).
If two or three animals die, testing at 5 mg/kg b.w. should be performed.
If fewer than two animals die, the test item should be retested (second step) with 50 mg/kg b.w., using three animals of the same sex.
If, in this second step, two or three animals die, testing at 5 mg/kg b.w. should be performed. If, in this second step, no to one animal dies, no further testing is necessary.
Results and discussion
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 300 - < 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- yes, at 2000 mg/kg bw. all animals died prematurely
- Clinical signs:
- other: Under the present test conditions, a single oral administration of 300 mg 3-Methylpyrazole/kg b.w. did not reveal any signs of toxicity. No premature death was recorded within the test period. No signs or abnormalities were noted at necropsy. A single ora
- Gross pathology:
- No signs or abnormalities were noted at necropsy.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- In this study 3-Methylpyrazole was examined for acute toxicity after a single oral administration to rats.
Under the present test conditions, a single oral administration of 300 mg 3-Methylpyrazole/kg b.w. did not reveal any signs of toxicity. No premature death was recorded within the test period. No signs or abnormalities were noted at necropsy.
A single oral administration of 2000 mg 3-Methylpyrazole/kg b.w. revealed reduced motility, ataxia, reduced muscle tone, dyspnoea and dorsal position in all 3 of 3 animals and lateral position in 2 animals. All animals died prematurely. No signs or abnormalities were noted at necropsy.
All surviving animals gained the expected body weight.
The LD50 value was ranked between 300 and 2000 mg/kg b.w. - Executive summary:
In this study 3-Methylpyrazole was examined for acute toxicity after a single oral administration to rats. Under the present test conditions, a single oral administration of 300 mg 3-Methylpyrazole/kg b.w. did not reveal any signs of toxicity. No premature death was recorded within the test period. No signs or abnormalities were noted at necropsy. A single oral administration of 2000 mg 3-Methylpyrazole/kg b.w. revealed reduced motility, ataxia, reduced muscle tone, dyspnoea and dorsal position in all 3 of 3 animals and lateral position in 2 animals. All animals died prematurely. No signs or abnormalities were noted at necropsy. All surviving animals gained the expected body weight. The LD50 value was ranked between 300 and 2000 mg/kg b.w.
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