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EC number: 222-720-6
CAS number: 3586-55-8
test substance in the test medium:
Sampling time [h]
Nominal Conc. [mg/L]
Measured Conc. [mg/L]
results in the table were not corrected by the recoveries obtained for the
analytical method. A mean recovery of 101% was determined.
test solutions showed no significant loss of the test substance.
Therefore, nominal concentrations can be considered as effective
of main test:
During the test period, the
temperature (measured at 0 h and 48 h) was within a total range
stretching from 20.0 to 22.5 °C and varied in some single vessels by up
Results of main
95 % c.l.
24 h [mg/L]
48 h [mg/L]
and E100 were directly taken from the concentrations
being tested. E50(48h) calculated as the geometric mean
between E0 and E100.
tested for acute toxicity towardsthe
water flea Daphnia magna according
to the OECD Guideline 201 part I.
animals were exposed to 5 concentrations of the test substance, ranging
from 10 to 100 mg/l, and a control group. The test was performed at 20.0
– 22.5 °C in a medium according to DIN 38412, part 11. Immobilisation
was assessed at 24 and 48 hours, physico-chemical water parameters and
formaldehyde concentrations were measured at the beginning and after 48
test substance was
found to be acutely harmful for Daphnia
magna. All results refer to the nominal concentrations since the
formaldehyde concentration was found stable within the incubation
concentration-response relationship can be established from the study
effects were observed at concentrations from 40 mg/L and above. Because
of the steep concentration-effects curve after 48 h, the EC50 was
calculated as the geometric mean between EC0 and EC100 representing
28 mg/L of the test substance.
test substance EGForm hydrolyses
rapidly and completely in the test medium, forming ethylene glycol and
formaldehyde. Formaldehyde is expected to dominate the toxicity of
EGForm in aqueous solutions. Dissolved Formaldehyde
is expected to be non-volatile, and no losses due to adsorption are to
of the formaldehyde concentrations during the test proved that no
significant losses occurred. Therefore, the effective concentrations can
be considered to be equal to the nominal concentrations.
the test period, the temperature (measured at 0 h and 48 h) was within a
total range stretching from 20.0 to 22.5 °C and varied in some single
vessels by up to 2.4°C. According to the Guideline, the test temperature
should range between 18 and 22 °C and remain constant within ±1°C in
single tests. It is expected that this deviation does not signifcantly
influence the results.
toxicity towards invertebrates was tested with the water flea Daphnia
magna. An EC50 (48h) of 28 mg/L was derived.
For ethylene glycol a 48 h EC50 value to
Daphnia magna was determined to 46300 mg/L.
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