3-aminopropylmethylamine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

OECD Guideline 306 (Biodegradability in Seawater)

Principles of method if other than guideline:

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance (3-aminopropyl)(methyl)amine. The study was performed according to OECD Guideline 306 (Biodegradability in Seawater).

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: (3-aminopropyl)(methyl)amine
- Common name: 3-Amino-1-methylaminopropane
- Molecular formula: C4H12N2
- Molecular weight: 88.1528 g/mol
- Smiles notation: N(CCCN)C
- InChl: 1S/C4H12N2/c1-6-4-2-3-5/h6H,2-5H2,1H3
- Substance type: Organic
- Physical state: Liquid
- Purity: > 95%
- Other: The test chemical was obtained commercially (Sigma-aldrich or Fluka). No further purification was necessary before testing.

Oxygen conditions:

not specified

Inoculum or test system:

other: Seawater

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): The seawater was collected from an expected non-polluted location (90 m depth in the Trondheimsfjord, Norway).
- Storage conditions: Seawater was stored in dark conditions in 10 L tanks at 20 ± 2°C.
- Storage length: Seawater was stored for 2-5 days.
- Water filtered: Yes, inoculum was collected and filtered (50 µm).

Duration of test (contact time):

28 d

Initial conc.:

2 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

other: BOD

Details on study design:

TEST CONDITIONS
- Composition of medium: Aged and fortified seawater was used as a test medium for the study.
- Additional substrate: Additional nutrient solutions were added to the test medium.
- Test temperature: 20 ± 2°C
- Continuous darkness: No data available

TEST SYSTEM
- Culturing apparatus: Stoppered bottles were used as a test vessel for the study. Each bottle was completely filled without air bubbles present.
- Number of culture flasks/concentration: Duplicates
- Measuring equipment: The concentrations of dissolved oxygen (DO) in separate bottles were measured with a probe connected to a dissolved oxygen meter (YSI Instruments, YSI Inc., Yellow Springs, OH). The content of each bottle was discarded after measurement.

SAMPLING
- Sampling frequency: Sampling was performed at the start of the experiment and after 5, 15 and 28 days, respectively.
- Sampling method: Bottles in each series with blank, test and reference solutions were removed for measurements at day 0, 5, 15 and 28, during the incubation period.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Aged and fortified seawater without any test or reference chemical was used as a blank sample.

Reference substance:

aniline

Remarks:

Aniline (diluted to 2 mg/l in the aged and nutrient-fortified seawater) was used as a reference substance for the study.

Key result

Parameter:

other: BOD

Value:

70.9

Sampling time:

28 d

Remarks on result:

other: Other details not known

Details on results:

Test substance undergoes 70.9% degradation by BOD parameter in 28 days.

Table: Experimental results of compounds tested for biodegradability.

 

 

Compound	Biodegradability
	ThODNH3	BOD28 (% of ThOD)
3-Amino-1-methylaminopropane (MAPA)	2.00	70.9

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

The percentage degradation of test substance (3-aminopropyl)(methyl)amine was determined to be 70.9% by BOD parameter in 28 days. Thus, based on percentage degradation, (3-aminopropyl)(methyl)amine is considered to be readily biodegradable in water.

Executive summary:

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance (3-aminopropyl)(methyl)amine (CAS no. 6291 -84 -5). The study was performed according to OECD Guideline 306 (Biodegradability in Seawater).The test chemical was obtained commercially (Sigma-aldrich or Fluka) with purity of >95%. No further purification was necessary before testing. Seawater was used as a test inoculum for the studycollected from an expected non-polluted location (90 m depth in the Trondheimsfjord, Norway)and filtered (50µm).Seawater was stored in dark conditions for 2-5 days in 10 L tanks at 20 ± 2°C.The aged seawater was aerated for 20 min and fortified with nutrient solutions enhancing bacterial growth. Test solutions were prepared by diluting the compound tested to 2 mg/l. Aged and fortified seawater was used as a test medium for the study. Additional nutrient solutions were added to the test medium.Aniline (diluted to 2 mg/l in the aged and nutrient-fortified seawater) was used as a reference substance for the study. Aged and fortified seawater without any test or reference chemical was used as a blank sample.The solutions were distributed as duplicates in bottles with stoppers,each bottle was completely filled without air bubbles present. The bottles were incubated for upto 28 days at 20 ± 2°C.Bottles in each series with blank, test and reference solutions were removed for measurements at day 0, 5, 15 and 28, during the incubation period. The concentrations of dissolved oxygen (DO) in separate bottles were measured with a probe connected to a dissolved oxygen meter (YSI Instruments, YSI Inc., Yellow Springs, OH). The content of each bottle was discarded after measurement. During each measurement the temperature in one of the blank bottles was recorded with the temperature sensor of the oxygen probe. The BOD values were calculated as the difference in DO between blank solution and the test solutions. BOD was calculated as mg BOD/mg test solution. The final results were given as percentage BOD relative to the theoretical oxygen demand (ThOD) calculated under the assumption that nitrogen is eliminated as ammonia.The percentage degradation of test substance(3-aminopropyl)(methyl)aminewas determined to be70.9% by BOD parameter in 28 days.Thus, based on percentage degradation, (3-aminopropyl)(methyl)amine is considered to be readily biodegradable in nature.  

Description of key information

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance (3-aminopropyl)(methyl)amine (CAS no. 6291 -84 -5)

(Ingvild Eide-Haugmo et. al; 2012). The study was performed according to OECD Guideline 306 (Biodegradability in Seawater).The test chemical was obtained commercially (Sigma-aldrich or Fluka) with purity of >95%. No further purification was necessary before testing. Seawater was used as a test inoculum for the studycollected from an expected non-polluted location (90 m depth in the Trondheimsfjord, Norway)and filtered (50µm).Seawater was stored in dark conditions for 2-5 days in 10 L tanks at 20 ± 2°C.The aged seawater was aerated for 20 min and fortified with nutrient solutions enhancing bacterial growth. Test solutions were prepared by diluting the compound tested to 2 mg/l. Aged and fortified seawater was used as a test medium for the study. Additional nutrient solutions were added to the test medium.Aniline (diluted to 2 mg/l in the aged and nutrient-fortified seawater) was used as a reference substance for the study. Aged and fortified seawater without any test or reference chemical was used as a blank sample.The solutions were distributed as duplicates in bottles with stoppers,each bottle was completely filled without air bubbles present. The bottles were incubated for upto 28 days at 20 ± 2°C.Bottles in each series with blank, test and reference solutions were removed for measurements at day 0, 5, 15 and 28, during the incubation period. The concentrations of dissolved oxygen (DO) in separate bottles were measured with a probe connected to a dissolved oxygen meter (YSI Instruments, YSI Inc., Yellow Springs, OH). The content of each bottle was discarded after measurement. During each measurement the temperature in one of the blank bottles was recorded with the temperature sensor of the oxygen probe. The BOD values were calculated as the difference in DO between blank solution and the test solutions. BOD was calculated as mg BOD/mg test solution. The final results were given as percentage BOD relative to the theoretical oxygen demand (ThOD) calculated under the assumption that nitrogen is eliminated as ammonia.The percentage degradation of test substance(3-aminopropyl)(methyl)aminewas determined to be70.9% by BOD parameter in 28 days.Thus, based on percentage degradation, (3-aminopropyl)(methyl)amine is considered to be readily biodegradable in nature.  

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

Various experimental studies and predicted data for the target compound (3-aminopropyl)(methyl) amine (CAS No. 6291-84-5) were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental key study from peer reviewed journal (Ingvild Eide-Haugmo et. al; 2012), biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance (3-aminopropyl)(methyl)amine (CAS no. 6291 -84 -5). The study was performed according to OECD Guideline 306 (Biodegradability in Seawater).The test chemical was obtained commercially (Sigma-aldrich or Fluka) with purity of >95%. No further purification was necessary before testing. Seawater was used as a test inoculum for the study collected from an expected non-polluted location (90 m depth in the Trondheimsfjord, Norway)and filtered (50µm).Seawater was stored in dark conditions for 2-5 days in 10 L tanks at 20 ± 2°C.The aged seawater was aerated for 20 min and fortified with nutrient solutions enhancing bacterial growth. Test solutions were prepared by diluting the compound tested to 2 mg/l. Aged and fortified seawater was used as a test medium for the study. Additional nutrient solutions were added to the test medium. Aniline (diluted to 2 mg/l in the aged and nutrient-fortified seawater) was used as a reference substance for the study. Aged and fortified seawater without any test or reference chemical was used as a blank sample. The solutions were distributed as duplicates in bottles with stoppers, each bottle was completely filled without air bubbles present. The bottles were incubated for upto 28 days at 20 ± 2°C.Bottles in each series with blank, test and reference solutions were removed for measurements at day 0, 5, 15 and 28, during the incubation period. The concentrations of dissolved oxygen (DO) in separate bottles were measured with a probe connected to a dissolved oxygen meter (YSI Instruments, YSI Inc., Yellow Springs, OH). The content of each bottle was discarded after measurement. During each measurement the temperature in one of the blank bottles was recorded with the temperature sensor of the oxygen probe. The BOD values were calculated as the difference in DO between blank solution and the test solutions. BOD was calculated as mg BOD/mg test solution. The final results were given as percentage BOD relative to the theoretical oxygen demand (ThOD) calculated under the assumption that nitrogen is eliminated as ammonia. The percentage degradation of test substance (3-aminopropyl)(methyl)amine was determined to be70.9% by BOD parameter in 28 days. Thus, based on percentage degradation, (3-aminopropyl)(methyl)amine is considered to be readily biodegradable in nature.  

 

In a supporting study, biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance (3-aminopropyl)(methyl)amine (CAS no. 6291 -84 -5)(Ingvild Eide-Haugmo et. al; 2009). The study was performed according to OECD Guideline 306 (Biodegradability in Seawater). The test chemical was diluted in normal seawater which had been aged for 3-5 days, and essential nutrients for the bacteria in the seawater were added. Seawater without chemical was used for blank samples. Aniline (diluted in sea water) was used as a reference substance for the study. The solutions were distributed as duplicates in bottles with stoppers, and incubated in the dark for 28 days at 20 ± 2°C. A dissolved oxygen meter was used to measure the concentration of dissolved oxygen in solution.At the start of the experiment and after 5, 15 and 28 days bottles in each series with blank, test and reference solutions were removed for measurements. After measurement the bottles were discharged. The biological oxygen demand (BOD) was used as a measure for biodegradation. The BOD values were calculated as the difference in dissolved oxygen between solution with blank and test solutions, and the percentage of the theoretical oxygen demand (ThOD) was calculated. The percentage degradation of test substance (3-aminopropyl)(methyl)amine was determined to be 70% by BOD parameter in 28 days. Thus, based on percentage degradation, (3-aminopropyl) (methyl)amine is considered to be readily biodegradable in nature.

 

Another biodegradation study (from authoritative database J-CHECK, 2017) was conducted for 28 days for evaluating the percentage biodegradability of test substance (3-aminopropyl) (methyl)amine (CAS no. 6291-84-5). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance (3 -amino propyl)(methyl)amine was determined to be 89, 95 and 100% by BOD(NH3), TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation, (3 -amino propyl)(methyl)amine is considered to be readily biodegradable in nature.

 

In a prediction using the Estimation Programs Interface Suite (EPI suite, 2017), the biodegradation potential of the test compound(3-aminopropyl)(methyl) amine(CAS No. 6291-84-5) in the presence of mixed populations of environmental microorganisms was estimated.The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical (3-aminopropyl)(methyl)amine is expected to be readily biodegradable.

 

On the basis of above results for target chemical(3-aminopropyl)(methyl) amine(from peer reviewed journals and authoritative database J-CHECK & EPI suite, 2017), it can be concluded that the test substance (3-aminopropyl)(methyl) amine can be expected to be readily biodegradable in nature.