1,5-dimethyl-1-vinylhept-4-enyl acetate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March - November 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No CoA. No water solubility data or vapour pressure data. Study was performed in the dark (acceptable deviation), still concentrations declined over time. No description of analytical method. No description of validity criteria. Use of final concentrations is considered worst case to conclude effects.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Deviations:

yes

Remarks:

test was performed in darkness.

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 3.2, 5.8, 10, 18, 32 mg/L
- Sampling method: at 0 and 48 hours composite samples of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels.
- Sample storage conditions before analysis:kept at - 18 °C to - 22°C until analysis.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The stock solution was prepared by mixing 99.8 mg test substance with 400 mL water by polytron for 5 minutes and made up to 1000 mL with water. Calculated mounts of the stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed. The daphnia were then transferred into the beakers. The test substance was homogeneously distributed in the test vessels at all test times and test concentrations.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water Flea
- Strain/clone: STRAUS 1820
- Source: CIBA-GEIGY Ltd.
- Feeding during test: None during the test.
- Food type: A suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract.
- Amount: in such quantities that the food is consumed after 24h
- Frequency: 3x/week

ACCLIMATION
- Acclimation period: 24 hours before the begin of the exposure reproductive daphnia are separated from the young by sieving all individuals through a 800 µm sieve. This operation is repeated immediately before the exposure and the young (O-24h of age) are retained for the test.
- Breeding conditions (same as test or not): Cultures of daphnia are maintained in glass vessels containing approx. 2.5L of reconstituted water at 20 ± 1 °C (water is renewed partially thrice weekly). At each renewal the daphnia are fed.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

240 mg CaCO3/L

Test temperature:

21 °C

pH:

t=0h 7.8
t=48h 7.7 - 7.9

Dissolved oxygen:

t=0h 99-101%
t=48h 98 - 100%

Nominal and measured concentrations:

Nominal: 0, 3.2, 5.8, 10, 18, and 32 mg/L
Measured t=0h: <0.1, 2.4, 4.5, 7.4, 14.4, and 30.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers
- Type: closed, covered with watch glases
- Fill volume: 100 mL
- Aeration: The water was aerated with clean air for at least 24 hours before use
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water containing 65 mg NaHCO3, 294 mg CaCl2 * 2H2O, MgSO4 * 7H2O, 6 mg KCL 1000 mL bidistilled water.

OTHER TEST CONDITIONS
- Photoperiod: The test substance is reported to be unstable when exposed to light. Therefore the exposure of the daphnia was done in darkness.

EFFECT PARAMETERS MEASURED:
Immobilizations were recorded after 24 and 48 hours exposure.

VEHICLE CONTROL PERFORMED: not applicable

RANGE-FINDING STUDY: not applicable

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: calculated and graphically determined

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Observed value

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

6.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Observed value

Details on results:

Calculated results:
EC50 (48h): 2.8 mg/L
95% confidence limit : 2.5 - 3.1 mg/L
Goodness of fit CHI: 0.0

EC50 (24h): 3.4 mg/L
95% confidence limit : 2.9 - 3.9 mg/L
Goodness of fit CHI: 11.6

Values graphically determined: EC50: (48h): 2.8 mg/L

Values observed:
NOEC (48h): 1.0 mg/L
EC0 (48h): 1.0 mg/L
EC100 (48h): 6.6 mg/L

Controls, immobilizations in blank: 0

*Remark: The test substance is reported to be unstable when exposed to light. Therefore the exposure of the daphnia was done in darkness. As only 31-43% of the nominal concentration could be found at the end of exposure the EC and NOEC values are based on measured concentrations at 48 h.

Reported statistics and error estimates:

Calculations:
EC-values were graphically determined on gausso-logarithmic probability paper.
The LC-50/EC-50 values were calculated according to the maxirnun likelihood method, probit model (Mc Cullagh, P., Nelder, J.A., 1983: Generalized linear models, Chapman & Hall, London)

48h		# daphnia	immobilized
actual conc.  (mg/L)	replicate 1	2	3	4	total	%
blank	0	0	0	0	0	0
1	0	0	0	0	0	0
2.5	1	1	2	1	5	25
3.7	5	4	5	5	19	95
6.6	5	5	5	5	20	100
10.9	5	5	5	5	20	100

Validity criteria fulfilled:

not specified

Remarks:

however, oxygen levels and control mortality can be concluded to fulfill the validity criteria.

Conclusions:

Under the conditions of the test, the 48h EC50 value for Daphnids was determined to be 2.8 mg/L (based on t = 48h measured concentrations)

Executive summary:

An acute aquatic toxicity test was performed according to OECD TG 202 (1984) and in compliance with GLP. 20 Daphnia magna (4 replicates, 5 organisms per test vessel) were exposed in a static system to nominal concentrations of 0, 3.2, 5.8, 10, 18, 32 mg/L. The test substance was reported to be unstable when exposed to light. Therefore the exposure of the daphnia was done in darkness. Measured 48h concentrations were <0.1, 1.0, 2.5, 3.7, 6.6, and 10.9 mg/L. Observations regarding mobility of the Daphnids were made at 24 and 48 hours. Based on final measured concentrations the 48-hour EC50 was calculated and graphically determined to be 2.8 mg/L. The 48 -hour NOEC and EC100 were calculated to be 1.0 mg/L and 6.6 mg/L, respectively.

 

Description of key information

An acute aquatic toxicity test was performed according to OECD TG 202 (1984) and in compliance with GLP. 20 Daphnia magna (4 replicates, 5 organisms per test vessel) were exposed in a static system to nominal concentrations of 0, 3.2, 5.8, 10, 18, 32 mg/L. The test substance was reported to be unstable when exposed to light. Therefore the exposure of the daphnia was done in darkness. Measured 48h concentrations were <0.1, 1.0, 2.5, 3.7, 6.6, and 10.9 mg/L. Observations regarding mobility of the Daphnids were made at 24 and 48 hours. Based on final measured concentrations the 48-hour EC50 was calculated and graphically determined to be 2.8 mg/L. The 48 -hour NOEC and EC100 were calculated to be 1.0 mg/L and 6.6 mg/L, respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

2.8 mg/L