Tar, wood

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 2018-01-28 til 2018-02-15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

TOC analysis

Details on sampling:

- Concentrations:
- Sampling method:
- Sample storage conditions before analysis:

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: WAF according to OECD Guideline 293

- Evidence of undissolved material (e.g. precipitate, surface film, etc.): Yes, surface, column, sediment. Test solution have therefore been filtered before use.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Nominal and measured concentrations:

4.5, 10, 22, 45 and 100 mg/L Loading before filtering the test solutions

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

ca. 31 mg/L

Nominal / measured:

nominal

Conc. based on:

other: TOC

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

NOELR

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Conc. based on:

other: TOC

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities:
- Observations on body length and weight:
- Other biological observations:
- Mortality of control:
- Other adverse effects control:
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
- Effect concentrations exceeding solubility of substance in test medium:

Validity criteria fulfilled:

yes

Conclusions:

All validity criteria fulfilled.

Executive summary:

Within a valid GLP conform study the substance Tar, wood, has been been assessed for its toxicity in fish, rainbow trout (Oncorhynchus mykiss), according to the OECD protocol 203/ EU method C.1.

Validity was assessed by a tox control and confirmed by the quality control of the laboratory.

NOEC was determined to be 22 mg/L/96 hrs,

LOEC was determined to be 45 mg/L/96 hrs and

LL50 was determined to be 31 mg/L/96 hrs

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

31 mg/L

Additional information

Study is valid and used for the ES calculations.