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Short-term toxicity to aquatic invertebrates

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Description of key information

The following results were determined for the test item Tar wood (species: Daphnia magna). 

24h-NOEC = 20 mg/L
48h-NOEC = 20 mg/L
24h-EC50i = 37 mg/L
48h-EC50i = 28 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
37 mg/L

Additional information

The study was performed using five concentrations ranging from 10 to 100 mg/L nominal load. Twenty Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

The concentrations showed toxicity between 0 and 95% immobilisation. None of the animals were immobilised in the control.

For the estimation of the EC50s the fits showed sufficient statistical correspondence of the data with the dose-response-equation.

At the beginning and at the end of the test, the content of the test item in the test solutions was estimated using DOC-determination. The measured concentrations were in the range of 38 % - 76 % of the nominal concentration. Therefore, the determination of the biological results was based on the geometric mean of the measured concentrations.

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study (201308R201). The value was determined as 1.4 mg/L, lying within the demanded range of 0.6 – 1.7 mg/L.

No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met. 

The result of the test is considered valid.