2,4,6-tris(2,4,6-tribromophenoxy)-1,3,5-triazine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and appropriate guidelines

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EC Council directive 67/548/EEC, as last amended by Commission Directive 92/69/EEC, Annex V, Part B, Methods for the determination of Toxicity, B.3: "Acute Toxicity-Dermal". Official Journal of the European Communities No L 383, 1992

Principles of method if other than guideline:

not relevant

GLP compliance:

yes

Test type:

fixed dose procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,4,6-tris(2,4,6-tribromophenoxy)-1,3,5-triazine

EC Number:

426-040-2

EC Name:

2,4,6-tris(2,4,6-tribromophenoxy)-1,3,5-triazine

Cas Number:

25713-60-4

Molecular formula:

C 21 H 6 Br 9 N 3 O 3

IUPAC Name:

tris(2,4,6-tribromophenoxy)-1,3,5-triazine

Constituent 2

Reference substance name:

4260402

IUPAC Name:

4260402

Details on test material:

Identification SR-245
Description White powder
Batch 969066
Purity 100%
Test substance storage At room temperature in the dark
Stability under storage conditions Stable

Test animals

Species:

rat

Strain:

other: Wistar strainCr1:(WI) BR (outbred, SPF-Quality)

Sex:

male/female

Details on test animals or test system and environmental conditions:

number of animals: 5 males and 5 females (females were nulliparous and non pregnant)
Age and body weight Young adult animals (approx. 8 weeks old) were selected. Body weight variation did not exceed +/- 20% of the sex means.
Identification Earmark
Husbandary:
Air conditioned room with approx 15 air changes/hr
Temp: 21 Deg C.
RH: 50%
Light: 12 hr artificial light 12 hr dark.
Accommodation: 5 animals per sex per cage.
Acclimatisation period was at least 5 days before start of the treatment
Diet Standard pelleted laboratory animal diet
Water Free access to tap water

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

other: 1% Aqueous carboxymethyl cellulose

Details on dermal exposure:

24 hr, after which dressings were removed and residual test substance removed using a tissue moistened with tap water

Duration of exposure:

24 h

Doses:

2000 mg/kg (10 ml/kg) body weight

No. of animals per sex per dose:

5 males and 5 females

Control animals:

not specified

Details on study design:

Mortality/viability Twice daily
Body weights Days 1 (pre-administration), 8 and 15
Clinical signs At periodic intervals on the day of dosing (day 1) and once daily thereafter, until day 15. Time of onset, degree and duration were recorded and symptoms graded according to fixed scales.
Necropsy At the end of the observation period, all animals were sacrificed by asphyxiation using oxygen/carbon dioxide procedure and subjected to necropsy. Descriptions of all internal macroscopic abnormalities were recorded.

Statistics:

No statistical analysis was performed

Results and discussion

Preliminary study:

n/a

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

Male: 2000 mg/kg bw; Number of animals: 5; Number of deaths: 0
Female: 2000 mg/kg bw; Number of animals: 5; Number of deaths: 0

Clinical signs:

other: Signs of toxicity related to dose levels: No deaths occurred and no signs of toxicity were observed.

Gross pathology:

Effects on organs:
No treatment-related macroscopic findings were observed.

Other findings:

Signs of toxicity (local):
Red staining of the snout was noted in one female, on day 2.

Any other information on results incl. tables

See attached files on tables and figures

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The dermal LD50 value of SE-245 in rats was established as exceeding 2000 mg/kg body weight.

Executive summary:

n/a