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EC number: 204-111-7 | CAS number: 115-84-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 72 hours, 27 to 30 June 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline and GLP-compliant study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-Butyl-2-ethyl-1,3-propanediol
- IUPAC Name:
- 2-Butyl-2-ethyl-1,3-propanediol
- Reference substance name:
- 2-butyl-2-ethylpropanediol
- EC Number:
- 204-111-7
- EC Name:
- 2-butyl-2-ethylpropanediol
- Cas Number:
- 115-84-4
- Molecular formula:
- C9H20O2
- IUPAC Name:
- 2-butyl-2-ethylpropane-1,3-diol
- Details on test material:
- - Name of test material (as cited in study report): BEPD
- Physical state: colourless solid
- Analytical purity: 99.5%
- Lot/batch No.: HRC accession number 20.4.95/1
- Expiration date of the lot/batch: 31 December 1995.
- Storage condition of test material: in darkness at room temperature (ca. 20 degrees C).
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken at 0 hours (fresh media) and 72 hours (expired media).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test material was dispersed directly in sterile nutrient medium with the aid of unltrasonic disruption for 30 minutes.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- - Strain number: CCAP 278/4
- Source: Culture Centre of Algae & Protozoa c/o Freshwater Biological Association, Cumbria, UK.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Not applicable
Test conditions
- Test temperature:
- 23 °C
- pH:
- 7.3 - 8.1
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal: 2.2, 4.6, 10, 22, 46 and 100 mg/L
Measured: 2.2, 4.6, 9.8, 21, 45 and 100 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 94 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks:
- (area under the growth curve, EbC50)
- Remarks on result:
- other: 95% CL: 83 - 106 mg BEPD/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 45 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Williams' test (1971, 1972) was used to compare the percentage inhibition in each treated group with the baseline (control) values. Bartlett's test for homogeneity of variance (1937) was also applied.
Any other information on results incl. tables
Results of the definitive test are compiled below:
Nominal BEPD concentration (mg/L) |
Mean BEPD concentration (mg/L) |
Mean cell counts (cells x 10000/ml) |
||||||
0 hours |
72 hours |
Mean |
Mean as % of nominal |
0 hours |
24 hours |
48 hours |
72 hours |
|
Control |
ND |
ND |
- |
- |
0.99 |
3.7 |
12 |
59 |
2.2 |
2.121 |
2.230 |
2.2 |
99 |
0.99 |
3.2 |
12 |
57 |
4.6 |
4.450 |
4.800 |
4.6 |
101 |
1.0 |
3.8 |
31 |
65 |
10 |
9.400 |
10.10 |
9.8 |
98 |
0.97 |
3.8 |
12 |
59 |
22 |
20.08 |
21.22 |
21 |
94 |
1.0 |
3.8 |
13 |
60 |
46 |
43.07 |
47.09 |
45 |
98 |
0.99 |
3.6 |
11 |
53 |
100 |
98.13 |
103.6 |
100 |
101 |
0.99 |
3.0 |
6.2 |
25 |
ND: None detected
E: Expired media
Nominal BEPD concentration (mg/L) |
Mean measured concentration mg BEPD/L |
Area under curve at 72 h |
% Inhibition* |
Growth rate (0 - 72 h) |
% Inhibition* |
Control |
Control |
1018 |
0 |
0.057 |
0 |
2.2 |
2.2 |
981 |
4 |
0.056 |
1 |
4.6 |
4.6 |
1137 |
<12> |
0.058 |
<2> |
10 |
9.8 |
1040 |
<2> |
0.057 |
<1> |
22 |
21 |
1062 |
<4> |
0.057 |
0 |
46 |
45 |
936 |
8 |
0.055 |
3 |
100 |
100 |
460 |
55 |
0.045 |
21 |
* Percentage inhibition values calculated using non-rounded data
< > Increase in algal growth
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72-hour ErC50 (growth rate), EbC50 (integrated biomass) for S. capricornutum exposed to BEPD under static conditions were >100 and 94 mg BEPD/L, respectively.
- Executive summary:
S. capricornutum was exposed to BEPD at nominal concentrations of 2.2, 4.6, 10, 22, 46 and 100 mg/L in a static 72 -hour toxicity test carried out according to OECD 201 guidelines. The test design included an untreated control comprising untreated algal growth medium only. Concentrations of BEPD were verified analytically at test initiation and termination and measured concentrations were 91 - 98% of nominal. The 72 -hour ErC50 and EbC50 endpoints determined were >100 and 94 mg BEPD/L, respectively. The corresponding 72 -hour NOEC based on inhibition of specific growth rate, determined using Williams' test analysis, was 45 mg BEPD/L.
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