Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Specific investigations: other studies

Currently viewing:

Administrative data

biochemical or cellular interactions
Type of information:
experimental study
Adequacy of study:
other information
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference Type:
Acetaldehyde-mediated cilia dysfunction in bovine bronchial epithelial cells
Sisson J.H.
Bibliographic source:
Am J Physiol;260(2 Pt 1):L29-36.

Materials and methods

Principles of method if other than guideline:
Ciliated bovine bronchial epithelial cells obtained from fresh bronchi were tested for their beating function after exposure to isobutyraldehyde in an election ATP-activated axonemes and an ATP-ase activity assay.
GLP compliance:
not specified
Type of method:
in vitro
Endpoint addressed:
not applicable

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
- Name of test material: Isobutyraldehyde

Test animals

other: bovine bronchial epithelial cells

Administration / exposure

Details on study design:
Ciliated bovine bronchial epithelial cells and fresh bovine trachea obtained from a local slaughterhouse. Actively beating ciliated cells were observed, and there motion was quantified by measuring cilia beat frequency using phase-contrast microscopy and videotape analysis. The axoneme motion after ATP-reactivation was qualitatively assessed by comparing the motion of the test substance-exposed axonemes to that of buffer-exposed axonemes. Acetaldehyde binding to cilia proteins was determined using electrophoretic and autoradiotraphic analysis of acetaldehyde-labeled cilia proteins. ATPase activity was determined by measuring the liberation of 32-P-labeled inorganic phosphate from [γ 32P]ATP.

Results and discussion

Details on results:
The test substance dose-dependently inhibited the cilia beating and the cilia ATPase activity in bovine bronchial epithelial cells.

Applicant's summary and conclusion