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EC number: 201-149-6 | CAS number: 78-84-2
Specific investigations: other studies
Administrative data
- Endpoint:
- biochemical or cellular interactions
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Acetaldehyde-mediated cilia dysfunction in bovine bronchial epithelial cells
- Author:
- Sisson J.H.
- Year:
- 1 991
- Bibliographic source:
- Am J Physiol;260(2 Pt 1):L29-36.
Materials and methods
- Principles of method if other than guideline:
- Ciliated bovine bronchial epithelial cells obtained from fresh bronchi were tested for their beating function after exposure to isobutyraldehyde in an election ATP-activated axonemes and an ATP-ase activity assay.
- GLP compliance:
- not specified
- Type of method:
- in vitro
- Endpoint addressed:
- not applicable
Test material
- Reference substance name:
- Isobutyraldehyde
- EC Number:
- 201-149-6
- EC Name:
- Isobutyraldehyde
- Cas Number:
- 78-84-2
- Molecular formula:
- C4H8O
- IUPAC Name:
- 2-methylpropanal
- Details on test material:
- - Name of test material: Isobutyraldehyde
Constituent 1
Test animals
- Species:
- other: bovine bronchial epithelial cells
Administration / exposure
- Details on study design:
- Ciliated bovine bronchial epithelial cells and fresh bovine trachea obtained from a local slaughterhouse. Actively beating ciliated cells were observed, and there motion was quantified by measuring cilia beat frequency using phase-contrast microscopy and videotape analysis. The axoneme motion after ATP-reactivation was qualitatively assessed by comparing the motion of the test substance-exposed axonemes to that of buffer-exposed axonemes. Acetaldehyde binding to cilia proteins was determined using electrophoretic and autoradiotraphic analysis of acetaldehyde-labeled cilia proteins. ATPase activity was determined by measuring the liberation of 32-P-labeled inorganic phosphate from [γ 32P]ATP.
Results and discussion
- Details on results:
- The test substance dose-dependently inhibited the cilia beating and the cilia ATPase activity in bovine bronchial epithelial cells.
Applicant's summary and conclusion
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