Registration Dossier
Registration Dossier
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EC number: 201-149-6 | CAS number: 78-84-2
Specific investigations: other studies
Administrative data
- Endpoint:
- biochemical or cellular interactions
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Acetaldehyde-mediated cilia dysfunction in bovine bronchial epithelial cells
- Author:
- Sisson J.H.
- Year:
- 1 991
- Bibliographic source:
- Am J Physiol;260(2 Pt 1):L29-36.
Materials and methods
- Principles of method if other than guideline:
- Ciliated bovine bronchial epithelial cells obtained from fresh bronchi were tested for their beating function after exposure to isobutyraldehyde in an election ATP-activated axonemes and an ATP-ase activity assay.
- GLP compliance:
- not specified
- Type of method:
- in vitro
- Endpoint addressed:
- not applicable
Test material
- Reference substance name:
- Isobutyraldehyde
- EC Number:
- 201-149-6
- EC Name:
- Isobutyraldehyde
- Cas Number:
- 78-84-2
- Molecular formula:
- C4H8O
- IUPAC Name:
- 2-methylpropanal
- Details on test material:
- - Name of test material: Isobutyraldehyde
Constituent 1
Test animals
- Species:
- other: bovine bronchial epithelial cells
Administration / exposure
- Details on study design:
- Ciliated bovine bronchial epithelial cells and fresh bovine trachea obtained from a local slaughterhouse. Actively beating ciliated cells were observed, and there motion was quantified by measuring cilia beat frequency using phase-contrast microscopy and videotape analysis. The axoneme motion after ATP-reactivation was qualitatively assessed by comparing the motion of the test substance-exposed axonemes to that of buffer-exposed axonemes. Acetaldehyde binding to cilia proteins was determined using electrophoretic and autoradiotraphic analysis of acetaldehyde-labeled cilia proteins. ATPase activity was determined by measuring the liberation of 32-P-labeled inorganic phosphate from [γ 32P]ATP.
Results and discussion
- Details on results:
- The test substance dose-dependently inhibited the cilia beating and the cilia ATPase activity in bovine bronchial epithelial cells.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
