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EC number: 201-149-6 | CAS number: 78-84-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
- Reference Type:
- publication
- Title:
- Salmonella Mutagenicity Tests: II. Results From Testing of 270 Chemicals
- Author:
- Mortelmans, K. et al.
- Year:
- 1 986
- Bibliographic source:
- Environ. Mutagen. 8, Suppl. 7, 1-119
- Reference Type:
- publication
- Title:
- Salmonella mutagenicity tests: V. Results from the testing of 311 chemicals
- Author:
- Zeiger E et al.
- Year:
- 1 992
- Bibliographic source:
- Environ. Mol. Mutagen . 19 (Suppl . 21), 2-141
Materials and methods
- Principles of method if other than guideline:
- Method: other: Haworth, S. et al.: Environ. Mutagen. 5, Suppl. 1, 3-142
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Isobutyraldehyde
- EC Number:
- 201-149-6
- EC Name:
- Isobutyraldehyde
- Cas Number:
- 78-84-2
- Molecular formula:
- C4H8O
- IUPAC Name:
- 2-methylpropanal
- Details on test material:
- - Name of test material (as cited in study report): Isobutyraldehyde
- Purity: 98%
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA97, TA98, TA100, TA102, TA1535, TA1537, and TA104
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rats and hamster S-9 mix (5, 10 or 30%)
- Test concentrations with justification for top dose:
- 0 - 10000 µg/plate depending on strain used
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 4-nitro-o-phenylenediamine and 2-aminoanthracene
- Remarks:
- See any other information on materials and methods incl. tables
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 minutes
- Exposure duration: 48 hours
NUMBER OF REPLICATIONS: 3 plates per dose level
DETERMINATION OF CYTOTOXICITY
- Method: Appearance of his- pinpoint colonies, reduced numbers of revertant colonies per plate, or thinning or absence of the bacterial lawn. - Evaluation criteria:
- A positive response is defined as a reproducible, dose-related increase in histidine-independent (revertant) colonies in any one strain/activation combination. An equivocal response is defined as an increase in revertants that is not dose related, not reproducible, or not of sufficient magnitude to support a determination of mutagenicity. A negative response is obtained when no increase in revertant colonies is observed following chemical treatment. There was no minimum percentage or fold increase required for a chemical to be judged positive or weakly positive.
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium TA104, TA97, TA98, TA100, TA102 TA1535, and TA1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- In the report of the NTP the results of TA104 with rat S9 mix is considered to be equivocal. However, Mortelmans et al does not consider the results to be equivocal. Based on the only slight increase in revertants the results cannot be considered to be equivocal, but are negative.
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