Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-167-4 | CAS number: 79-01-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Although the study was not carried out according to a guideline and GLP, it is well described and the result is considered acceptable.
- Principles of method if other than guideline:
- In this study, a new, closed system is used, in which a KHCO3/K2CO3 buffer to supply the algae with CO2 is employed, but the buffer is separated
from the test medium to avoid growth inhibition due to the ionic strength. - GLP compliance:
- no
- Analytical monitoring:
- yes
- Vehicle:
- not specified
- Details on test solutions:
- Saturated solutions in growth medium were prepared. Defined aliquots of these solutions were added to the medium.
- Test organisms (species):
- Chlamydomonas reinhardtii
- Details on test organisms:
- 7-day old precultures; cell density at start of exposure 5.103 cells/ml.
Strain number 11-32a SAG, from the University of Göttingen, Germany.
Precultures and test cultures were grown in the medium for unicellular algae according to Kuhl (1962). Incubation of all cultures was done in a Orbital Incubator (Gallenkamp). The cultures were shaken permanently with a frequency of 120 rpm. They were illuminated from above with 130 uE/m2s
without light-dark cycle. The photosynthetically effective light was determined with a Quantum Sensor from Licor Inc. - Test type:
- other: closed system
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
- Hardness:
- Not specified
- Test temperature:
- 20 ± 1 °C
- pH:
- 6.5-7.5
- Dissolved oxygen:
- Not specified
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Measured
- Details on test conditions:
- A CO2 buffer and the contaminated medium were filled into the test vessel at least 15 hours before the assay was started by adding the algal
inoculum. The inoculum was prepared from 7 day old precultures at a concentration of 3x105 cells/mL. For the test 0.5 mL inoculum was added to 30 mL medium. The cell density in the test cultures amounted to 5x103 cells/mL at the beginning of the assay. The test was run for 72 hours. After this period, the algae, which have multiplicated at least by factor 100 during the 72-h incubation time, were taken for determination of biomass. - Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 36.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI 35.1-38.2 mg/l
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 12.3 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI 9.76-14.3 mg/l
- Reported statistics and error estimates:
- The 72-h EC10 and 72-h EC50 values could be determined very precisely, as demonstrated by the small 95% confidence intervals.
Reference
Description of key information
For freshwater algae the lowest toxicity values were the 72-h EC50 of 36.5 mg/l and the 72-h EC10 of 12.3 found in Chlamydomonas reinhardtii.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 36.5 mg/L
- EC10 or NOEC for freshwater algae:
- 12.3 mg/L
Additional information
A cell multiplication test with Chlamydomonas reinhardii (algae) was available. Although not conducted according to a guideline the study was found to be acceptable as no major deviations were present (Brack and Rottler, 1994). The 72-h EC50 was 36.5 mg/l and the 72-h EC10 was 12.3 mg/l, these values are used in the assessment. Other studies were found to be less critical, not reliable or the information was not enough to assess validity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
