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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, well documented and acceptable for assessment.

Data source

Reference
Reference Type:
publication
Title:
Biotransformation of 12C- and 2-13C-Labeled Methyl tert-Butyl Ether, Ethyl tert-Butyl Ether, and tert-Butyl Alcohol in Rats: Identification of Metabolites in Urine by 13C Nuclear Magnetic Resonance and Gas Chromatography/Mass Spectrometry
Author:
Ulrike Bernauer
Year:
1998
Bibliographic source:
Chemical Research in Toxicology 1998, 11, 651-658

Materials and methods

Objective of study:
metabolism
Principles of method if other than guideline:
Rats were exposed to either tert-butyl alcohol at natural carbon isotope ratio or with 13C-enriched tert-butyl alcohol (250 mg/kg of body weight) to analyse the metabolism of tert-butyl alcohol. Urine was collected and the metabolites were identified by NMR and GC/MS.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylpropan-2-ol
EC Number:
200-889-7
EC Name:
2-methylpropan-2-ol
Cas Number:
75-65-0
Molecular formula:
C4H10O
IUPAC Name:
2-methylpropan-2-ol
Test material form:
other: liquid
Details on test material:
- Name of test material (as cited in study report): tert-butyl alcohol
- Analytical purity: 97 %
- Radiochemical purity (if radiolabelling): 97 %
Radiolabelling:
yes

Test animals

Species:
rat
Strain:
Fischer 344
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Harlan Winkelmann
- Weight at study initiation: 220-260 g
- Housing: single housing
- Individual metabolism cages: yes
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21°C
- Photoperiod (hrs dark / hrs light): 12/12 hours

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Duration and frequency of treatment / exposure:
once
Doses / concentrations
Remarks:
Doses / Concentrations:
250 mg/kg body weight
No. of animals per sex per dose / concentration:
3
Control animals:
no
Details on dosing and sampling:
METABOLITE CHARACTERISATION STUDIES
- Tissues and body fluids sampled (delete / add / specify): urine
- Time and frequency of sampling: for a time period of 72 hours
- From how many animals: (samples pooled or not) all 3 animals
- Method type(s) for identification: GC-MS, NMR

Results and discussion

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
tert-butyl alcohol, tert-butyl alcohol glucuronide, and tertbutyl alcohol sulfate, 2-hydroxyisobutyrate and 2-methyl-1,2-propanediol were identified as metabolites after treatment with tert-butyl alcohol.

Applicant's summary and conclusion