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Toxicity to microorganisms

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Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14 - 15 Oct 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Initial biomass concentration: extinction of 0.140 at a wave length of 436 nm
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Remarks on exposure duration:
16 +/- 1 hour
Test temperature:
21.8 °C
pH:
before incubation: 5.6 - 6.2
after incubation: 6.8
Nominal and measured concentrations:
nominal content of active substance: 313, 626, 1251, 2502.5, 5005 and 10010 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 mL Erlenmeyer flasks filled with 100 mL test medium
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

Reference substance (positive control):
no
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
> 10 g/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth inhibition
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
> 10 g/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth inhibition

Test Concentration [mg a.s./L]

Inhibition [%]

313

-6.9

626

-7.3

1251

-4.7

2502.5

-2.9

5005

-0.9

10010

7.4

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
23 Feb - 01 Mar 1993
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Does not meet important criteria of today standard methods
Qualifier:
according to guideline
Guideline:
other: DIN 38412 part 27
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min
Duration:
30 min
Dose descriptor:
EC10
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
not stated
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Only short abstract available
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Qualifier:
according to guideline
Guideline:
other: DIN 38412 part 12
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Pseudomonas putida
Test type:
static
Limit test:
no
Total exposure duration:
30 min
Duration:
30 min
Dose descriptor:
EC10
Effect conc.:
500 mg/L
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Description of key information

EC10 > 10 g/L based on growth inhibition

Key value for chemical safety assessment

Additional information

One reliable study on microorganisms is available for AES (C12-14, 1-2.5EO) Na (CAS 68891-38-3). In the GLP-study (Sasol, 1993d) on growth inhibition of Pseudomonas putida according to DIN 38412, part 8 an EC10 and an EC50 -values of > 10 g/L could be determined after approximately 16 h of exposure. This study is defined as key study. The two additions study available for microorganisms (BASF, 1993b and BASF, 1998d) are regarded as not reliable as test duration and given endpoints based on respiration rate do not meet today standard methods.