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Diss Factsheets
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EC number: 202-044-8 | CAS number: 91-15-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline with acceptable restrictions (missing E. coli/ TA102 strain)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- missing strain to detect crosslinking/ oxidizing agents
- Principles of method if other than guideline:
- According to Ames, B.N. et al.: Mutation Research 31, 347-364
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Phthalonitrile
- EC Number:
- 202-044-8
- EC Name:
- Phthalonitrile
- Cas Number:
- 91-15-6
- Molecular formula:
- C8H4N2
- IUPAC Name:
- benzene-1,2-dicarbonitrile
- Details on test material:
- - Name of test material (as cited in study report): phthalonitrile
- Analytical purity: purity of the substance is not documented
Constituent 1
Method
- Target gene:
- nis-
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S-9 mix
- Test concentrations with justification for top dose:
- 3.15, 10, 31.5, 100, 315, 1000 and 3000 µg/plate (with S-9 mix); 10, 31.5, 100, 315, 1000 and 3000 µg/plate (without S-9 mix)
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: see freetext
- Details on test system and experimental conditions:
- Standard plate test
Test tubes containing 2 mL soft agar kept in a water bath at 45°C, and remaining
components added in the following order:
0 .1 mL test solution or vehicle
0 .1 mL bacterial suspension
0 .5 mL S-9 mix (in tests with metabolic activation) or 0 .5 ml KCl (in tests
without metabolic activation).
After mixing, the samples are poured onto Vogel-Bonner agar plates.
Where indicated the epoxide hydratase inhibitor and glutathione depletor 1, 1, 1 -trichloropropene 2,3-oxide (TCPO) was added together with the top agar (0.6/µL per plate) .
Two test plates per dose and controls were used; after incubation at 37°C for
48-72hours in the dark, the bacterial colonies ( his+ revertants) are counted.
Positive control:
with metabolic activation: 10 μg/plate Benzo(a)pryene, 10 μg/plate 2-aminoanthracene and 90 μg/plate 3-Methylcholanthrene for each strain;
without metabolic activation: 1 μg/plate Benzo(a)pryene-4,5-oxide and 3.15 μg/plate N-MethyI-N'-nitro-N-nitrosoguanidine, all substances were dissolved in DMSO for all strains.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- At the two highest concentrations the test compound partly precipitated on the plate .
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'. Remarks: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537
Any other information on results incl. tables
Standard plate test (3.15 - 3000 µg/plate) |
|||||
Strain |
Metabolic activation system |
Replicates |
maximum revertant factor |
dose dependency |
Assessment |
TA 98 |
no |
2 |
1.2 |
no |
negative |
|
yes |
2 |
1.1 |
no |
negative |
TA 98 |
not performed |
|
|
|
|
with TCPO |
yes |
2 |
1.1 |
no |
negative |
TA 100 |
no |
2 |
1.0 |
no |
negative |
Trial 1 |
yes |
2 |
1.0 |
no |
negative |
TA 100 |
not performed |
|
|
|
|
Trial 2 |
yes |
2 |
1.0 |
no |
negative |
TA 1535 |
no |
2 |
1.3 |
no |
negative |
|
yes |
2 |
1.3 |
no |
negative |
TA 1537 |
no |
2 |
1.1 |
no |
negative |
|
yes |
2 |
1.2 |
no |
negative |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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