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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
26/10/2010 - 26/01/2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 306 (Biodegradability in Seawater)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dissolved in seawater
- Final dilution of a dissolved solid, stock liquid or gel: 5.2 mg/ L
- Final preparation of a solid: Dissolved in seawater

FORM AS APPLIED IN THE TEST (if different from that of starting material) : aqueous, in solution
Oxygen conditions:
aerobic
Inoculum or test system:
natural water: marine
Details on inoculum:
- Source of inoculum: Natural sea water pumped from Flotta (Scapa Flow), pumped continuously from a depth of 2 meters below low water Spring tide level, salinity 34 - 37 ‰,
- Storage conditions: 20 000 L storage tank. 5-7 days prior to test initiation transferred to ageing tank and stored in darkness
- Initial cell/biomass concentration: at collection 1.25 x 10 (to the power of 2)
- Water filtered: yes
- Type and size of filter used, if any: gravity filtered to 45 µM
Duration of test (contact time):
28 d
Initial conc.:
7.09 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: Natural seawater
- Additional substrate: Reference substance sodium benzoate (positive control)
- Test temperature: 19.1 - 20.4 °C
- pH: 7.82 - 7.92
- pH adjusted: no
- Aeration of dilution water: during pretreatment for 20 minutes
- Continuous darkness: yes, during 5-7 days pretreatment

TEST SYSTEM
- Measuring equipment: polarographic electrode
- Test performed in open system: No, performed in closed system

SAMPLING
- Sampling frequency: Measurements taken on day 7, 14, 21 and 28 days
- Sampling method: Measuring DOC, BOC and inhibition due to test material
- Sample storage before analysis: 5-7 days in an ageing tank in darkness

CONTROL AND BLANK SYSTEM
- Inoculum blank: Background oxygen consumption in seawater (0 mg/ L test material)
- Toxicity control: seawater containing test material and sodium benzoate and seawater containing only sodium benzoate
Reference substance:
benzoic acid, sodium salt
Test performance:
2-Butylbutanoic acid compound with 2 aminoethanol was 88% degraded at 7 days, 95% degraded at 14 days, 98% degraded at 21 days and 100% biodegraded by 28 days.
Parameter:
% degradation (DOC removal)
Value:
88
Sampling time:
7 d
Parameter:
% degradation (DOC removal)
Value:
95
Sampling time:
14 d
Parameter:
% degradation (DOC removal)
Value:
98
Sampling time:
21 d
Key result
Parameter:
% degradation (DOC removal)
Value:
100
Sampling time:
28 d
Key result
Parameter:
COD
Value:
1.862 mg O2/g test mat.
Results with reference substance:
- > 60% biodegradation of ThOD in 14 days
- Reference substance degradation was also measuring by BOD and percentage degradation
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The study showed that 2-butylbutanoic acid compound with 2-aminoethanol biodegraded by 100% over 28 days and had an inhibition of 21% to seawater bacteria
Executive summary:

The study assesses the ready biodegradability of 2-butylbutanoic acid compound with 2-aminoethanol in seawater. The study is compliant with GLP and follows the OECD guideline 306: 'Biodegradability in Seawater - Closed Bottle Method'. All validity criteria were complied with. Testing used natural seawater which was filtered and pretreated with a 5 -7 day period in an ageing tank in darkness. The BOD was 4.56, 4.92, 5.07 and 5.34 mg O2/ L for 7, 14, 21 and 28 days, respectively for the definitive test (1 replicate per time period). An oxygen consumption control was included with a BOD of 0.4, 0.5, 0.88 and 1.29 mg O2/ L for 7, 14, 21 and 28 days, respectively. Two positive controls were present (reference material, sodium benzoate), one with the reference material only dissolved in seawater (BOD was 1.74, 2.05, 1.89 and 1.92 mg O2/ L for 7, 14, 21 and 28 days, respectively) and the other with both the test material and reference material (BOD was 5.56, 6.07, 6.13 and 5.71 mg O2/ L for 7, 14, 21 and 28 days, respectively). The definitive test showed that the 2 -butylbutanoic acid compound with 2 -aminoethanol in seawater degraded by 88% by 7 days, 95% by 14 days, 98% by 21 days and 100% by 28 days.

Description of key information

The ready biodegradability of 2-butylbutanoic acid compound with 2-aminoethanol was assessed with a GLP study in accordance with the OECD guideline 306: 'Biodegradability in Seawater - Closed Bottle Method'. All validity criteria were complied with. Testing used natural seawater which was filtered and pre-treated with a 5 -7 day period in an ageing tank in darkness. The BOD was 4.56, 4.92, 5.07 and 5.34 mg O2/ L for 7, 14, 21 and 28 days, respectively for the definitive test (1 replicate per time period). An oxygen consumption control was included with a BOD of 0.4, 0.5, 0.88 and 1.29 mg O2/ L for 7, 14, 21 and 28 days, respectively. Two positive controls were present (reference material, sodium benzoate), one with the reference material only dissolved in seawater (BOD was 1.74, 2.05, 1.89 and 1.92 mg O2/ L for 7, 14, 21 and 28 days, respectively) and the other with both the test material and reference material (BOD was 5.56, 6.07, 6.13 and 5.71 mg O2/ L for 7, 14, 21 and 28 days, respectively). The test showed that the 2 -butylbutanoic acid compound with 2 -aminoethanol in seawater degraded by 88% at day 7, 95% at day 14, 98% at day 21 and 100% at day 28. Therefore, the registered substance is deemed to be ready biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
marine water

Additional information