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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
GLP compliance:
yes (incl. QA statement)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Secondary effluent of city sewage treatment plant
- Preparation of inoculum for exposure: Sludge filtered through filter paper (No 2)
- Pretreatment: None
Duration of test (contact time):
28 d
Initial conc.:
7.39 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Solubilising agent (type and concentration if used): None used
- Test temperature: 19 - 21 ˚C
- pH adjusted: No
- Continuous darkness: Yes

TEST SYSTEM
- Number of culture flasks/concentration: 10 - 12
- Test performed in closed vessels due to significant volatility of test substance: Yes (sealed containers, test substance concentration verified analytically)

SAMPLING
- Sampling frequency: day 0, 7, 14, 21, and 28

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control: No

STATISTICAL METHODS: None used
Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
28 d
Remarks on result:
other: also verified test substance concentration
Results with reference substance:
Sodium benzoate (the reference substance) attained a maximum level of biodegradation (based on the BOD) of 75 and 89% after 14 days.
Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
HFO-1234ze is not readily biodegradable.

Description of key information

The test substance is concluded to be "not biodegradable" under the conditions of the test.

Key value for chemical safety assessment

Biodegradation in water:
not biodegradable

Additional information

HFO-1234ze is not readily biodegradable after 28 days under aerobic conditions.